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Chemical Compound Review:

AC1L3GGB N-[2-[2-(3-aminopropylamino) ethyldisulfany...

Synonyms: AC1Q7ECM, NSC358533, AR-1K5409, WR 33278, 58205-87-1, ...

Liu, S.C. et al., Savoye, C. et al., Smoluk, G.D. et al., Mitchell, J.L. et al., Shigematsu, N. et al.

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High impact information on WR 33278

To better identify and develop potential intermediate biomarkers for chemoprevention at the molecular level we applied the technique of differential display RT-PCR to assess the effects of both the thiol (SH), i.e. WR1065 and the disulfide (SS), i.e. WR-33278, metabolites of amifostine on gene expression in CHO-AA8 cells [1].
High levels of the aminothiol WR-1065 protect cells from ionizing radiation, while much lower levels of this compound or its disulfide, WR-33278, impart anti-mutagenic effects [2].
WR-33278 (Z = +4) protects by scavenging of OH. and, in the case of the supercoiled plasmid DNA, by reducing the accessibility of radiolytic attack sites via the induction of packaging of DNA in liquid-crystalline condensates (observed by circular dichroism) [3].
The binding of WR-33278 (0.05-0.4 mM) to calf thymus DNA (6 mM, with respect to DNA phosphate) was determined at 50, 100, and 150 mM KCl in 1 mM Tris, pH 7, by equilibrium dialysis [4].
Cell survival was only enhanced following electroporation of 0.01 mM of spermine and WR-33278 30 min prior to irradiation [5].

References

Repression of c-myc gene expression by the thiol and disulfide forms of the cytoprotector amifostine. Liu, S.C., Murley, J.S., Woloschak, G., Grdina, D.J. Carcinogenesis (1997) [Pubmed]
Involvement of the polyamine transport system in cellular uptake of the radioprotectants WR-1065 and WR-33278. Mitchell, J.L., Judd, G.G., Diveley, R.R., Choe, C.Y., Leyser, A. Carcinogenesis (1995) [Pubmed]
Thiol WR-1065 and disulphide WR-33278, two metabolites of the drug ethyol (WR-2721), protect DNA against fast neutron-induced strand breakage. Savoye, C., Swenberg, C., Hugot, S., Sy, D., Sabattier, R., Charlier, M., Spotheim-Maurizot, M. Int. J. Radiat. Biol. (1997) [Pubmed]
Equilibrium dialysis studies of the binding of radioprotector compounds to DNA. Smoluk, G.D., Fahey, R.C., Ward, J.F. Radiat. Res. (1986) [Pubmed]
Protection against radiation-induced mutagenesis at the hprt locus by spermine and N,N"-(dithiodi-2,1-ethanediyl)bis-1,3-propanediamine (WR-33278). Shigematsu, N., Schwartz, J.L., Grdina, D.J. Mutagenesis (1994) [Pubmed]

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