Disease relevance of AIDS-123102

• Oligopeptidase B was overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein, purified using metal-affinity chromatography and crystallized using the hanging-drop vapour-diffusion technique in 7%(w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7 [1].

High impact information on AIDS-123102

• Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries [2].
• Two broad-zone experimental approaches, membrane-confined electrophoresis and analytical ultracentrifugation, were used to examine the electrostatic and electrohydrodynamic characteristics of aqueous solutions of bovine RNase A in the presence of 100 mM KCl and 10 mM Bis-Tris propane over a pH range of 6.00-8.00 [3].
• The preparation of cell-free extract in Bis-Tris propane/HCl, pH 7, buffer containing 0.025% (w/v) Triton X-100 and 15 mM MgCl(2) provided optimum conditions for the stabilization of FPP [4].
• Phosphodiester hydrolysis by lanthanide complexes of bis-tris propane [5].
• To determine the origin of this effect, we measured reaction kinetics in buffers of different chemical nature and concentration: Tris, bis-Tris propane, Tes, Hepes, and cacodylate [6].

Associations of AIDS-123102 with other chemical compounds

• The analyses are performed using 10 mmol/l HCl + 20 mmol/l L-histidine + 0.1% hydroxypropylmethyl cellulose (pH 6.0), 5 mmol/l MES, and 25 mmol/l MES + 10 mmol/l bis-tris-propane (pH 6.6) which served as leading, terminating and background electrolyte, respectively [7].
• Glycine (25mM) adjusted to pH 9.0 by bis-Tris-propane served as the running electrolyte system [8].
• In order to solve the structure using the MAD phasing method, crystals of the L-selenomethionine-substituted epimerase were grown in the presence of 20% PEG 3350, 0.2 M Na2SO4 and 0.1 M bis-Tris propane pH 8.5 [9].

Analytical, diagnostic and therapeutic context of AIDS-123102

• Potentiometric titrations of the mixtures of lanthanide(III) perchlorates and bis-Tris propane (BTP) reveal formation of dinuclear hydroxo complexes M2(BTP)2(OH)n(6-n), where M = La(III), Pr(III), Nd(III), Eu(III), Gd(III), and Dy(III) and n = 2, 4, 5, or 6, in the pH range 7-9 [5].

References