High impact information on 4-methoxynaphthalen-2-amine

• The method is based on the sequential cleavage of the synthetic peptide substrate, glutaryl-alanyl-alanyl-phenylanyl-4-methoxy-2-naphthylamide, by NEP and exogenous aminopeptidase M to yield free 4-methoxy-2-naphthylamine (MNA) [1].
• Assay of dipeptidyl peptidase IV in serum by fluorometry of 4-methoxy-2-naphthylamine [2].
• The reaction is stopped with citrate (100 mmol/L, pH 4.0) and the released 4-methoxy-2-naphthylamine is measured fluorometrically [2].
• The histochemical distribution of the thiol proteases cathepsin B and dipeptidyl peptidase I and the serine protease dipeptidyl peptidase II was examined in rat bone and joint using amino acid derivatives of 4-methoxy-2-naphthylamine (MNA) [3].
• Investigation of proteinases in the digestive tract using 4-methoxy-2-naphthylamine (MNA) substrates [4].

Biological context of 4-methoxynaphthalen-2-amine

• 2-Naphthylamine, 4-methoxy-2-naphthylamine, 7-amino-4-methylcoumarin and 7-amino-4-trifluoromethylcoumarin and X peptides have become a useful tool to study proteases by a battery of complementary methods; they now include biochemistry, section biochemistry, location cytochemistry, ultracytochemistry and isoelectric focusing [5].

Associations of 4-methoxynaphthalen-2-amine with other chemical compounds

• We have demonstrated proteinase activity in unfixed cells grown on tissue culture plates with a technique using 5-nitrosalicylaldehyde and peptide derivatives of 4-methoxy-2-naphthylamine [6].

Gene context of 4-methoxynaphthalen-2-amine

• Dipeptidyl peptidase II was visualized using either lysyl-prolyl or lysyl-alanyl derivatives of 4-methoxy-2-naphthylamine (MNA) as synthetic substrates at pH 5.5 and employing the osmiophilic coupler hexazotized pararosanaline (HXP) for localization [7].
• The cytochemical localization of acrosin (EC 3.4.21.10) was investigated using a fluorescent technique, based on the use of fluorogenic substrates, derivatives of 4-methoxy-beta-naphthylamine, which liberate a fluorescent tag that, if coupled to 5-nitrosalicylaldehyde, generates an insoluble, fluorescent product [8].

Analytical, diagnostic and therapeutic context of 4-methoxynaphthalen-2-amine

• Using synthetic 4-methoxy-2-naphthylamine substrates and fluorescence-band detection techniques after isoelectric focusing, an increase in the activity of endopeptidases was demonstrated [9].

References