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Chemical Compound Review

AC1MIXFR     [(2R,3S,4R,5R)-5-(2-amino-6- oxo-3H-purin-9...

Synonyms: Caged GTP, FT-0664196, 124830-99-5
 
 
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High impact information on Caged GTP

  • Crystals of Ha-Ras p21 with caged GTP at the active site have been used to investigate the conformational changes of p21 on GTP hydrolysis [1].
  • Complete x-ray data sets have been obtained for the starting caged-GTP state and the final GDP state after photolysis and hydrolysis, demonstrating the feasibility of time-resolved structural investigations of the process of GTP hydrolysis [2].
  • The GTP binding domain of the c-Ha-ras protooncogene product (p21'c) and the corresponding region from an oncogenic mutant form of the protein in which glycine at position 12 has been replaced by valine [p21'(G12V)] have been crystallized with P3-1-(2-nitro)phenylethylguanosine 5'-O-triphosphate (caged GTP) at their active sites [2].
  • X-ray crystal structure analysis of the catalytic domain of the oncogene product p21H-ras complexed with caged GTP and mant dGppNHp [3].
  • The timecourse for activation of ICa by photolysis of caged-GTP was markedly altered by intracellular application of either GDP beta S or GTP gamma S. Upon maximal stimulation of ICa by intracellular dialysis with cAMP, photoreleased GTP induced a small, rapid increase in ICa followed by a gradual inhibition [4].
 

Biological context of Caged GTP

 

Anatomical context of Caged GTP

 

Associations of Caged GTP with other chemical compounds

  • The use of pure diastereomers of caged GTP complexed to the more stable p21(G12P)' mutant of p21 and the addition of n-octyl-beta-D-glucopyranoside improved the reproducibility and decreased the mosaicity of the crystals significantly [7].
  • Rapid release of photolabile guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), tetralithium salt, by flash photolysis of intracellular caged-GTP gamma S increased the magnitude of the L-type calcium current: an effect independent of the ultraviolet flash per se or the production of photolytic by-products [6].
 

Gene context of Caged GTP

  • Using caged GTP the intrinsic and GAP catalyzed GTPase activity of H-ras p21 is studied [8].
 

Analytical, diagnostic and therapeutic context of Caged GTP

  • A lag period was also observed when ICa was stimulated by flash photolysis of caged GTP gamma S and when adenylyl cyclase was activated directly by rapid perfusion with forskolin [9].
  • As an example of such investigations, the time-resolved FTIR studies on the GTPase reaction of H-ras p21 using caged GTP is presented [10].

References

  1. Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis. Schlichting, I., Almo, S.C., Rapp, G., Wilson, K., Petratos, K., Lentfer, A., Wittinghofer, A., Kabsch, W., Pai, E.F., Petsko, G.A. Nature (1990) [Pubmed]
  2. Biochemical and crystallographic characterization of a complex of c-Ha-ras p21 and caged GTP with flash photolysis. Schlichting, I., Rapp, G., John, J., Wittinghofer, A., Pai, E.F., Goody, R.S. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  3. X-ray crystal structure analysis of the catalytic domain of the oncogene product p21H-ras complexed with caged GTP and mant dGppNHp. Scheidig, A.J., Franken, S.M., Corrie, J.E., Reid, G.P., Wittinghofer, A., Pai, E.F., Goody, R.S. J. Mol. Biol. (1995) [Pubmed]
  4. Activation of L-type Ca2+ currents in cardiac myocytes by photoreleased GTP. Kozlowski, R.Z., Goodstadt, L.J., Twist, V.W., Powell, T. Proc. Biol. Sci. (1992) [Pubmed]
  5. Microtubule assembly and oscillations induced by flash photolysis of caged-GTP. Marx, A., Jagla, A., Mandelkow, E. Eur. Biophys. J. (1990) [Pubmed]
  6. Flash photolysis of intracellular caged GTP gamma S increases L-type Ca2+ currents in cardiac myocytes. Kozlowski, R.Z., Twist, V.W., Brown, A.M., Powell, T. Am. J. Physiol. (1991) [Pubmed]
  7. Crystallographic studies on p21(H-ras) using the synchrotron Laue method: improvement of crystal quality and monitoring of the GTPase reaction at different time points. Scheidig, A.J., Sanchez-Llorente, A., Lautwein, A., Pai, E.F., Corrie, J.E., Reid, G.P., Wittinghofer, A., Goody, R.S. Acta Crystallogr. D Biol. Crystallogr. (1994) [Pubmed]
  8. Molecular reaction mechanisms of proteins monitored by time-resolved FTIR-spectroscopy. Gerwert, K. Biol. Chem. (1999) [Pubmed]
  9. Rate-limiting steps in the beta-adrenergic stimulation of cardiac calcium current. Frace, A.M., Méry, P.F., Fischmeister, R., Hartzell, H.C. J. Gen. Physiol. (1993) [Pubmed]
  10. Fourier transform infrared photolysis studies of caged compounds. Cepus, V., Ulbrich, C., Allin, C., Troullier, A., Gerwert, K. Meth. Enzymol. (1998) [Pubmed]
 
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