Disease relevance of Kethoxal

• The reaction of Escherichia coli ribosomes with beta-ethoxy-alpha-ketobutyraldehyde (kethoxal) in a buffer containing 50--100 mM Tris.HCl at pH 7.4, 50 mM NH4Cl, and 5 mM Mg(OAc)2 readily released the 5S RNA from the ribosomes [1].
• Secondary structure of the 5' end of bacteriophage MS2 RNA Methoxyamine and kethoxal modification [2].
• Diminished virucidal activity of kethoxal against vesicular stomatitis virus pretreated with guanidinating reagent and proteases [3].

High impact information on Kethoxal

• RNase P RNA was modified with dimethylsulfate or kethoxal in the presence or absence of tRNA, and sites of modification were detected by primer extension [4].
• The effect of protein binding on the topography of the complex is reflected in the kethoxal reactivity of the RNA moiety [5].
• Under conditions of stoichiometric binding at 1 mM drug concentration in vitro, both drugs strongly protect 23S rRNA bases A2058 and A2451 from dimethyl sulphate and G2505 from kethoxal modification; G2061 is also weakly protected from kethoxal [6].
• The combination of dimethylsulfate, kethoxal, and 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluene sulfonate (CMCT) offers the full range of information on base-pairing and solvent exposure concerning the four more abundant ribonucleotides [7].
• Protection of ribosomal RNA from kethoxal in polyribosomes. Implication of specific sites in ribosome function [8].

Biological context of Kethoxal

• Further, since the sites of kethoxal modification within the RNA sequences of intact subunits are known, the task of identifying the components of individual ribonucleoprotein complexes should be considerably simplified [9].
• The binding site of streptomycin on the RNAs was determined via chemical probing with dimethylsulfate and kethoxal [10].
• Mass spectrometric investigation of protein alkylation by the RNA footprinting probe kethoxal [11].

Anatomical context of Kethoxal

• Glyoxal and kethoxal induced SCEs but not ERCs in human peripheral lymphocytes [12].
• Induction of four proteins in eukaryotic cells by kethoxal bis(thiosemicarbazone) [13].
• Kethoxal bis(thiosemicarbazone) induces the synthesis of four proteins (100 000, 70 000, 35 000 and 25 000 daltons) in normal chick embryo cells [13].

Associations of Kethoxal with other chemical compounds

• Nucleotide sequences around kethoxal-reactive guanine residues of 23S RNA in 50S ribosomal subunits have been determined [14].

Gene context of Kethoxal

• Using chemical modification with CMCT, kethoxal, DMS, DEPC, and lead, we probed the structure of the IGR in short, defined transcripts and in full-length RNA3 in vitro, in yeast extracts, and in whole yeast cells [15].
• Although some residues are found reactive toward dimethylsulphate and kethoxal in regions predicted to be unpaired by the phylogenetic secondary structure model of 4.5S RNA, generally the reactivity is low, and some residues in internal loops are not reactive at all [16].
• Modification of amino acids and bovine pancreatic ribonuclease A by kethoxal [17].

Analytical, diagnostic and therapeutic context of Kethoxal

• Yeast precursor ribosomal RNA. Molecular cloning and probing the higher-order structure of the internal transcribed spacer I by kethoxal and dimethylsulfate modification [18].
• Sequence determination of Gp-rich oligonucleotides by means of the Kethoxal modification [19].

References