Disease relevance of CCRIS 1789

• Azidoalanine mutagenicity in Salmonella: effect of homologation and alpha-methyl substitution [1].
• A Bacillus subtilis transformation system was used to investigate the possible direct effect of L-azidoalanine on DNA in vitro [2].
• The lack of mutagenic response for racemic or L-azidoalanine in hisG46 and E. coli WP2- was like that reported for azide and is consistent with similar modes of action for these agents [3].

High impact information on CCRIS 1789

• The results provide strong experimental evidence that azidoalanine is significantly (p<0.01) mutagenic to all stages of spermatogenesis in Drosophila melanogaster [4].
• Thus, L-azidoalanine failed to produce any DNA damage even in the absence of an excision repair mechanism [2].
• The biochemical mechanism(s) of azidoalanine-induced mutagenesis, however, is not known [1].
• Chinese hamster cells and normal human skin fibroblasts were treated with extracts from Salmonella typhimurium or Hordeum vulgare (barley) containing a crude mutagenic metabolite, as well as with synthetically produced azidoalanine [5].
• In addition, both cell lines expressed the enzyme O-acetylserine(thio)-lyase which is responsible for the conversion of azide to azidoalanine, the putative mutagenic intermediate [6].

Biological context of CCRIS 1789

• Structure-activity relationships of the azide metabolite, azidoalanine, in S. typhimurium [7].
• With the purified azidoalanine and the azide-treated extracts from Hordeum vulgare, there was a statistically significant increase in the frequency of sister chromatid exchanges observed in both Chinese hamster cells and human fibroblasts [5].

Associations of CCRIS 1789 with other chemical compounds

• The in vitro synthesized sodium azide mutagenic metabolite (azidoalanine) produced single-strand breaks and proteinase K-sensitive sites in isolated, germinating barley embryos [8].

Analytical, diagnostic and therapeutic context of CCRIS 1789

• The levels of mutagenic metabolite of azide (presumably azidoalanine), were determined by bioassay in the two azide-treated maize callus types and compared to levels of mutagenic metabolite in embryos isolated from azide-treated kernels [9].

References