Disease relevance of N-acetylornithine

• Ornithine delta-transaminase activity in Escherichia coli: its identity with acetylornithine delta-transaminase [1].
• The chromosomal acetylornithine deacetylase (argE) gene of Myxococcus xanthus was identified via homology to acetylornithine deacetylases from other bacterial species [2].
• Immunochemical studies on acetylornithine 5-aminotransferase from Pseudomonas aeruginosa [3].

High impact information on N-acetylornithine

• This novel protein structure provides a starting point for rational design of specific analogs that may be useful in combating human and plant pathogens that utilize acetylornithine transcarbamylase rather than ornithine transcarbamylase [4].
• Nitrogen metabolism in actinorhizal nodules of Alnus glutinosa: expression of glutamine synthetase and acetylornithine transaminase [5].
• Comparison of the predicted amino acid sequence of B. stearothermophilus aminoacylase with those of eubacterial acetylornithine deacylase, succinyldiaminopimelate desuccinylase, carboxypeptidase G2, and eukaryotic aminoacylase I suggests a common origin for these enzymes [6].
• Cloning the Escherichia coli K-12 argD gene specifying acetylornithine delta-transaminase [7].
• Studies of inhibition kinetics demonstrate that the two substrates, acetylornithine and ornithine, compete for the same active site of acetylornithine delta-transaminase; thus, the ornithine delta-transaminase activity in E coli is due to acetylornithine delta-transaminase and not to a separate specific ornithine delta-transaminase [1].

Chemical compound and disease context of N-acetylornithine

• The synthesis of acetylornithine delta-transaminase is induced by arginine and by rifampin in an arginine-inducible mutant of Escherichia coli W [8].

Biological context of N-acetylornithine

• Interestingly, total deletion of the genomic ARG7 ORF resulted in an arginine-leaky phenotype, indicating that yeast cells possess an alternative route for generating ornithine from acetylornithine [9].

Anatomical context of N-acetylornithine

• Human blood plasma contains a variable amount of acetylornithine, averaging 1.1 +/- 0.4 mumol/l (range 0.8--0.2 mumol/l) [10].

Gene context of N-acetylornithine

• The cDNA encoding for the protein was cloned based on its amino-terminal sequence and the amino acid sequence deduced from the determined nucleotide sequences of the cDNA exhibited homology to the enzymes belong to the ArgE/DapE/Acy1/Cpg2/YscS protein family (including acetylornithine deacetylase, carboxypeptidase and aminoacylase-1) [11].
• Additionally, using the knot detection algorithm on structures in the Protein Data Bank, a previously unreported deep trefoil knot was found in acetylornithine transcarbamylase [12].

References