The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

ARG7  -  glutamate N-acetyltransferase

Saccharomyces cerevisiae S288c

Synonyms: Arginine biosynthesis bifunctional protein ArgJ, mitochondrial, ECM40, Extracellular mutant protein 40, YM9916.01C, YMR062C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ECM40

  • Furthermore, Arg7p is both processed and active in Escherichia coli, a heterologous background, and is also cleaved in vitro when produced by coupled transcription/translation in a reticulocyte lysate [1].
  • The three-dimensional structure of the ternary complex, EF-Tu:GTP:Phe-tRNAPhe, involving Thermus aquaticus EF-Tu and yeast Phe-tRNA(Phe), shows that Arg7 is in a position which permits salt bridge formation with Asp284, thus binding the N-terminus tightly to domain 2 [2].

High impact information on ECM40

  • When artificially co-expressed from separate genes, the two subpeptides can complement an arg7 mutation; ornithine acetyltransferase activity is measurable [1].
  • Maturation of Arg7p occurs at threonine 215 (N-side), in the region most conserved among the 17 ornithine acetyltransferases characterized [1].
  • By Western immunoblotting, we show that Arg7p is also present as two subpeptides in isolated mitochondria, but that processing occurs before targeting to the mitochondria: deletion of the N-terminal leader peptide results in cytosolic accumulation of N-Arg7p, whereas C-Arg7p partially reaches the organelle by itself [1].
  • Variation of the central DNA base sequence reverses the two AT base pairs contacted in the minor groove by Arg7 of the alpha2 N-terminal arm without significantly altering the DNA binding affinity of the a1/alpha2 heterodimer [3].
  • An arsenate-resistant mutant AR3 of Chlamydomonas reinhardtii is a recessive mutant generated by random insertional mutagenesis using the ARG7 gene [4].

Biological context of ECM40


Associations of ECM40 with chemical compounds

  • We have cloned by functional complementation and characterized the yeast ARG7 gene encoding mitochondrial ornithine acetyltransferase, the enzyme catalyzing the fifth step in arginine biosynthesis [5].

Other interactions of ECM40

  • We have addressed the question of the bifunctionality of the eucaryotic enzyme, showing that overexpressed ARG7 can complement yeast arg2 and Escherichia coli argA mutations (affecting acetylglutamate synthase) [5].

Analytical, diagnostic and therapeutic context of ECM40


  1. The yeast ARG7 gene product is autoproteolyzed to two subunit peptides, yielding active ornithine acetyltransferase. Abadjieva, A., Hilven, P., Pauwels, K., Crabeel, M. J. Biol. Chem. (2000) [Pubmed]
  2. Mutational analysis of Escherichia coli elongation factor Tu in search of a role for the N-terminal region. Mansilla, F., Knudsen, C.R., Laurberg, M., Clark, B.F. Protein Eng. (1997) [Pubmed]
  3. Crystal structure of the MATa1/MATalpha2 homeodomain heterodimer in complex with DNA containing an A-tract. Li, T., Jin, Y., Vershon, A.K., Wolberger, C. Nucleic Acids Res. (1998) [Pubmed]
  4. Insertional mutagenesis in a homologue of a Pi transporter gene confers arsenate resistance on chlamydomonas. Kobayashi, I., Fujiwara, S., Shimogawara, K., Kaise, T., Usuda, H., Tsuzuki, M. Plant Cell Physiol. (2003) [Pubmed]
  5. Characterization of the Saccharomyces cerevisiae ARG7 gene encoding ornithine acetyltransferase, an enzyme also endowed with acetylglutamate synthase activity. Crabeel, M., Abadjieva, A., Hilven, P., Desimpelaere, J., Soetens, O. Eur. J. Biochem. (1997) [Pubmed]
  6. Sequence analysis of the ARG7 gene of Schizosaccharomyces pombe coding for argininosuccinate lyase. Expression of the gene in Saccharomyces cerevisiae. Loppes, R., Michels, R., Decroupette, I., Joris, B. Curr. Genet. (1991) [Pubmed]
WikiGenes - Universities