High impact information on Smyd1

• Using a modified subtractive hybridization method to identify specific genes that influence early cardiac development, we found that Bop is expressed specifically in cardiac and skeletal muscle precursors before differentiation of these lineages [1].
• These results indicate that m-Bop is essential for cardiomyocyte differentiation and cardiac morphogenesis [1].
• We show that m-Bop is a histone deacetylase-dependent transcriptional repressor [1].
• Targeted deletion of Bop in mice disrupted maturation of ventricular cardiomyocytes and interfered with formation of the right ventricle [1].
• These findings identify Bop as an essential downstream effector gene of MEF2C in the developing heart, and reveal a transcriptional cascade involved in development of the anterior heart field and its derivatives [2].

Biological context of Smyd1

• The t-BOP and t-ncb cDNAs in CTLs result from alternative splicing of a single primary transcript, whereas the Bop transcripts expressed in CTLs and in muscle appear to be transcribed from different promoters [3].
• The Bop gene adjacent to the mouse CD8b gene encodes distinct zinc-finger proteins expressed in CTLs and in muscle [3].
• Similar kinetics of induction and localization of m-Bop and skNAC during the induction of myogenesis in cultured C2C12 cells suggests a possible associated role for these proteins during this process [4].
• The present studies demonstrate that expression of the Bop gene in lymphocytes appears to be confined to CD8-positive cells, and that Bop gene expression is inducible by Con A [3].

Anatomical context of Smyd1

• The Bop promoter also directs transcription in the skeletal muscle lineage, but only cardiac expression is dependent on MEF2 [2].
• Expression of Bop gene transcripts was previously observed in several long term CTL lines and in thymus [3].
• Western blot analysis with a hamster anti-BOP mAb have detected the BOP protein in muscle cells and in COS 7 cells transfected with Bop cDNA constructs [3].

Physical interactions of Smyd1

• In the present studies, m-Bop was observed to interact with skNAC, a reported transcriptional activator specific to heart and skeletal muscle [4].

Other interactions of Smyd1

• m-Bop, a repressor protein essential for cardiogenesis, interacts with skNAC, a heart- and muscle-specific transcription factor [4].

References