High impact information on Ebf3

• In transgenic mice, both OE1 and OE2 are necessary for expressing the human prepro-orexin gene in the LHA and for repressing its expression in the medial regions of the hypothalamus [1].
• Here, we report the result of this experiment and describe the developmental expression pattern in the ventral midbrain of three of the isolated genes, HNF3alpha, synaptotagmin I, and Ebf3 [2].
• The strong conservation of the protein regions corresponding to the DNA binding and dimerisation domains previously defined in Ebf strongly suggests that Ebf2 and Ebf3 also constitute DNA sequence-specific transcription factors [3].
• In the uterus alone, OE2 produced a significant increase in the content of nucleic acids and also induced the accumulation of transferrin and beta-actin mRNAs [4].
• We have detected for the first time an induction of transferrin gene expression in the mammary gland in response to OE2, and these results support the view that the pattern of transferrin gene multi-modulated expression is tissue- and species-specific [4].

Biological context of Ebf3

• There are two phylogenetically conserved regions located 287 bp (orexin regulatory element (OE) 1) and 2.5 kb (OE2) upstream of the transcription initiation site of the human prepro-orexin gene [1].

Anatomical context of Ebf3

• The specific response of the transferrin gene to OE2 was not found in the liver or in the uterus [4].
• Our studies by dot blot analysis have demonstrated that multiple doses of 17 beta-oestradiol (OE2; 0.5 mg/kg per day for 3 days) elicit a specific 3.5-fold increase in the transferrin mRNA levels in the mammary glands of virgin rats [4].

References