Disease relevance of UL46

• In this study, it is shown that the UL46 protein of HSV type 2 (HSV-2) is affected strikingly by the presence of US3 PK [1].
• UL46 protein was undetectable in the extracellular virions of US3-deficient virus [1].
• Synthesis, subcellular localization and VP16 interaction of the herpes simplex virus type 2 UL46 gene product [2].
• We developed a rabbit polyclonal antiserum reactive against a recombinant 6x His-UL46 fusion protein expressed in Escherichia coli, and using this antiserum identified the UL46 gene product of herpes simplex virus type 2 (HSV-2) to be phosphoproteins with apparent molecular masses of 82-, 84-, and 86-kDa in infected Vero cells [2].
• Herpes simplex virus type 1 (HSV-1) was labeled by the fusion of the green fluorescent protein to a structural protein of its tegument (VP11/12), the product of gene UL46 [3].

High impact information on UL46

• CD4 T-cell responses to herpes simplex virus type 2 major capsid protein VP5: comparison with responses to tegument and envelope glycoproteins [4].
• Responses to eight HSV-2 glycoprotein, tegument, nonstructural, or capsid antigens were compared in 19 donors [4].
• No VP16, a major viral tegument protein, is transported to the cell nucleus in the presence of NP-1 [5].
• The UL46 gene encodes virion tegument phosphoproteins, the properties and functions of which are poorly understood [1].
• The capsid-tegument-associated ICP10 was phosphorylated and had PK activity in vitro and on Immobilon membranes [6].

Biological context of UL46

• Moreover, our data showed that the HSV-2 UL46 protein, when coexpressed with VP16, enhanced alpha4 promotor-regulated gene expression in a transient luciferase reporter assay, while the expression of the UL46 protein alone suppressed it [2].
• Although its kinetics of production and its progress of distribution were different from those of the major tegument protein VP16 (the UL48 gene product or alpha-trans-inducing factor (alphaTIF)), most of the UL46 protein colocalized with VP16 in the late phase of infection, and copurified with it in column chromatography [2].
• These results suggest that herpes simplex tegument proteins are processed for antigen presentation in vivo and are possible candidate compounds for herpes simplex vaccines [7].

Anatomical context of UL46

• Recognition of herpes simplex virus type 2 tegument proteins by CD4 T cells infiltrating human genital herpes lesions [7].

References