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Gene Review:

tRNA-Leu - tRNA

Kazachstania servazzii

Robinson, R.R. et al., Randerath, E. et al., Garcia-Cantalejo, J.M. et al., Leuker, C.E. et al., Dietrich, A. et al., et al.

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Disease relevance of tRNA-Leu

In striking contrast, deletion of the corresponding C-terminal domain of Escherichia coli LeuRS abolished aminoacylation of tRNALeu and also amino acid editing of mischarged tRNA molecules [1].

High impact information on tRNA-Leu

These two genes are assigned to be tRNALeu genes because of significant sequence homology with yeast tRNA3Leu, and secondary structure homology with yeast tRNA3Leu intervening sequence [2].
Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences [2].
The 5' flanking regions are only weakly homologous, but each set of isoacceptors contains short regions of strong homology approximately 20 nucleotides preceding the tRNA coding sequences: GCNTTTTG preceding tRNAIle genes; and GANTTTGG preceding tRNALeu genes [2].
Solution conformation of several free tRNALeu species from bean, yeast and Escherichia coli and interaction of these tRNAs with bean cytoplasmic Leucyl-tRNA synthetase. A phosphate alkylation study with ethylnitrosourea [3].
Only two analogues, gamma-hydroxyleucine and homocysteine are activated and transferred to tRNALeu where a post-transfer proofreading occurs [4].

Biological context of tRNA-Leu

The gene encodes a tRNALeu with the anti-codon AAG, and unlike the other nuclear eukaryotic tRNALeu (AAG) gene sequenced (from C. elegans), contains an intervening sequence of 27 bp [5].
C. albicans could readily be transformed with plasmids encoding leucyl-tRNA genes with the anticodons CAA and UAA (recognizing the codons UUG and UUA) and expression of the heterologous tRNALeu could be demonstrated by Northern RNA blotting [6].
It contains four new open reading frames which encode potential proteins of more than 99 amino acids, as well as the ypt53, tRNALeu and gsr moffenes [7].
Yeast tRNA Leu UAG. Purification, properties and determination of the nucleotide sequence by radioactive derivative methods [8].
Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames [7].

Associations of tRNA-Leu with chemical compounds

Exchanging the anticodon arm of tRNALeu but not the D- or T psi C-arm to that of tRNASer seriously affected the leucine accepting activity [9].

Analytical, diagnostic and therapeutic context of tRNA-Leu

A second major species of leucine tRNA, tRNA Leu UAG (formerly designated tRNA Leu CUA) was purified from baker's yeast in a three-step procedure entailing BD-cellulose chromatography in the presence and absence of Mg2+ and Sephadex G-100 gel filtration [8].

References

Functional divergence of a unique c-terminal domain of leucyl-tRNA synthetase to accommodate its splicing and aminoacylation roles. Hsu, J.L., Rho, S.B., Vannella, K.M., Martinis, S.A. J. Biol. Chem. (2006) [Pubmed]
Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences. Robinson, R.R., Davidson, N. Cell (1981) [Pubmed]
Solution conformation of several free tRNALeu species from bean, yeast and Escherichia coli and interaction of these tRNAs with bean cytoplasmic Leucyl-tRNA synthetase. A phosphate alkylation study with ethylnitrosourea. Dietrich, A., Romby, P., Maréchal-Drouard, L., Guillemaut, P., Giegé, R. Nucleic Acids Res. (1990) [Pubmed]
The proofreading of hydroxy analogues of leucine and isoleucine by leucyl-tRNA synthetases from E. coli and yeast. Englisch, S., Englisch, U., von der Haar, F., Cramer, F. Nucleic Acids Res. (1986) [Pubmed]
A leucine tRNA gene adjacent to the QA gene cluster of Neurospora crassa. Huiet, L., Tyler, B.M., Giles, N.H. Nucleic Acids Res. (1984) [Pubmed]
Toxicity of a heterologous leucyl-tRNA (anticodon CAG) in the pathogen Candida albicans: in vivo evidence for non-standard decoding of CUG codons. Leuker, C.E., Ernst, J.F. Mol. Gen. Genet. (1994) [Pubmed]
Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames. Garcia-Cantalejo, J.M., Boskovic, J., Jimenez, A. Yeast (1996) [Pubmed]
Yeast tRNA Leu UAG. Purification, properties and determination of the nucleotide sequence by radioactive derivative methods. Randerath, E., Gupta, R.C., Chia, L.L., Chang, S.H., Randerath, K. Eur. J. Biochem. (1979) [Pubmed]
The anticodon loop is a major identity determinant of Saccharomyces cerevisiae tRNA(Leu). Soma, A., Kumagai, R., Nishikawa, K., Himeno, H. J. Mol. Biol. (1996) [Pubmed]

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