Disease relevance of Hist1h1d

• Comparison of the mouse phosphorylation pattern to that in rat C-6 glioma cells showed differences for H1e and H1d [1].
• In contrast, two differentiated (endoderm-like) cell lines derived from teratocarcinomas lack H1b, and one also lacks H1d [2].

High impact information on Hist1h1d

• To determine whether one of these H1s, in particular, was responsible for the compensation present in H1(0-/-) mice, each of the three H1 knockout mouse lines was bred with H1(0) knockout mice to generate H1c/H1(0), H1d/H1(0), or H1e/H1(0) double-knockout mice [3].
• The protein kinase inhibitor staurosporine, shown to dephosphorylate histone H1 and down-regulate MMTV in cultured cells, appears only to completely dephosphorylate the H1.3 isoform [4].
• Most of the H1 histones, namely H1a, H1c, H1d/e, and H1(0), completely aggregate polynucleosomes (1.3 kilobase pairs, 6 nucleosomes) at 270-360 nM, corresponding to a molar ratio of six to eight H1 molecules per reconstituted nucleosome [5].
• In this system the wild type H1d promoter was 20-fold stronger than the H1t promoter [6].
• H1b is much less varied, while H1d and H1e are invariant by these electrophoretic criteria [7].

Biological context of Hist1h1d

• They also show that neither H1b nor H1d is essential for cell division [2].
• A high degree of nucleotide sequence identity was seen between the H1var.2, rat H1d and human H1b genes, even well beyond the coding region, indicating that these genes are likely homologues [8].
• Using transient transfection assays in mouse fibroblast NIH3T3 cells, we showed that rat H1d contains a downstream region (+21 to +116) that imparts a two- to threefold up-regulation of fused reporters [9].

Regulatory relationships of Hist1h1d

• The rat histone H1d gene has intragenic activating sequences that are absent from the testis-specific variant H1t [9].

Other interactions of Hist1h1d

• Two of the genes that have not been assigned previously to the five somatic H1 subtypes have been identified as encoding the H1b and H1d subtypes [10].

Analytical, diagnostic and therapeutic context of Hist1h1d

• The H1d and H3.2 Omega sequences bound similar and perhaps identical proteins by gel shift analysis [9].

References