Disease relevance of Hist1h1e

• The binding of all known linker histones, named H1a through H1e, including H1(0) and H1t, to a model chromatin complex based on a DNA fragment containing the mouse mammary tumor virus long terminal repeat promotor was systematically studied [1].
• Comparison of the mouse phosphorylation pattern to that in rat C-6 glioma cells showed differences for H1e and H1d [2].

High impact information on Hist1h1e

• Mammalian somatic cells contain a set of H1 linker-histone subtypes, H1 (0) and H1a to H1e, that bind to nucleosome core particles and to the linker DNA between nucleosomes [3].
• Mice contain at least seven nonallelic subtypes of H1, including the somatic variants H1a through H1e, the testis-specific variant H1t, and the replacement linker histone H1(0) [4].
• To further investigate the in vivo role of individual mammalian H1s in development, we generated mice lacking H1c, H1d, or H1e by homologous recombination in mouse embryonic stem cells [5].
• HP1 binds specifically to Lys26-methylated histone H1.4, whereas simultaneous Ser27 phosphorylation blocks HP1 binding [6].
• The linker histone H1.4 is methylated on Lys(26) (K26/H1.4), but the role of this methylation in downstream events remains unknown [6].

Biological context of Hist1h1e

• Specific inhibitory effect of H1e histone somatic variant on in vitro DNA-methylation process [7].
• We found, using this highly purified system, that H1e protein binds preferentially and cooperatively to the GC-rich region of the DNA [8].

Anatomical context of Hist1h1e

• Whole-mount in situ hybridization using cloned H1c and H1e cDNAs revealed that the mRNAs were present in the cytoplasm of oocytes and 1-cell embryos, in contrast to the sea urchin early embryo where they are sequestered in the cell nucleus [9].
• H1c and H1e from both cell lines show in the quiescent state a relatively high specific activity comparable with that of H1(0) [10].
• In vivo phosphorylation of the five histone H1 variants H1a-H1e including H1(0) in NIH 3T3 mouse fibroblasts was examined during the cell cycle by using a combination of HPLC techniques and conventional AU gel electrophoresis [2].

Associations of Hist1h1e with chemical compounds

• A triethylammonium methanephosphonate system proved efficacious for the separation of rat histone subtype H1c from H1e and a perchlorate/triethylammonium phosphate system for the analysis of chicken and mouse linker histones [11].

Other interactions of Hist1h1e

• Two others, H1d and H1e, are present in a second patch, while the H1b gene is at least 500 kb away in a patch containing 14 core histone genes [12].
• H1b is much less varied, while H1d and H1e are invariant by these electrophoretic criteria [13].

References