High impact information on Hspa1l

• Heat shock proteins play an important role in a large number of physiological processes and it is tempting to speculate that Hsc70t, which exhibits testis-specific expression, may be identical to Orch-1 [1].
• We found Hsc70t, a testis-enriched variant of the Hsp70 family of heat shock proteins which is specifically expressed in post-meiotic germ cells, in the olfactory epithelium of mouse and human [2].
• The Hsp70 homolog gene, Hsc70t, is expressed under translational control during mouse spermiogenesis [3].
• Immunohistochemistry and in situ hybridization techniques were used to examine the precise localization of the Hsc70t product during the various stages of spermatogenesis [3].
• An antisense RNA probe generated from the 3' untranslated region of Hsc70t cDNA detected Hsc70t mRNA in late round spermatids from step 7 onward with the signal disappearing in spermatids at step 15 [3].

Biological context of Hspa1l

• The transcription initiation site of the Hsc70t gene was located at ca. 600 bp upstream of the heat-inducible Hsp70.3 gene, linked head-to-head [4].
• It is possible that this silencer may not only be involved in testis-specific expression of Hsc70t but also function in down-regulation of Hsp70.3 induction [5].
• Thus, Hsc70t mRNA first appears after meiosis in haploid cells but is not translated effectively until these cells progress to the transcriptionally inactive stage which coincides with chromatin condensation [3].

Anatomical context of Hspa1l

• The Hsc70t gene is a Hsp70 homolog gene expressed constitutively in spermatids in mice [4].
• Here, we have utilized immunohistochemistry with anti-mouse Hsc70t antiserum to examine the expression of the spermatid-specific Hsp70 antigen in the testes of several mammalian species with different degrees of testes migration [6].

Other interactions of Hspa1l

• The mouse Hsp70.3 gene encodes the major inducible HSP70 protein and is located in close proximity to the Hsc70t gene, a testis-specific variant of the Hsp70 gene family [5].
• Two additional genes encode proteins that are developmentally regulated and expressed specifically in spermatogenic cells (Hsp70-2, Hsc70t) [7].

Analytical, diagnostic and therapeutic context of Hspa1l

• A rabbit antiserum raised againstthe mouse Hsc70t-lacZ fusion protein detected the Hsc70t protein in the late spermatid-enriched fraction after two-dimensional Western blot analyses [3].

References