Disease relevance of Igk-V28

• Immunoglobulin kappa light chain variable region gene complex organization and immunoglobulin genes encoding anti-DNA autoantibodies in lupus mice [1].
• Moreover, we have analyzed the N1 progeny of a (B6 X C58) X C58 backcross to determine the segregation of the Mtv-8 provirus with respect to polymorphisms in the Igk-VSer and Igk-J loci [2].
• Thus, during the development of B lymphocytes, the commitment of immunoglobulin kappa light chain gene expression seems to be associated with the presence of DNase I hypersensitivities that reflect changes of chromosomal structure surrounding the single copy C kappa gene [3].

High impact information on Igk-V28

• We show here that the promoter for an immunoglobulin kappa light-chain gene also is strongly specific for lymphoid cells [4].
• Expression of an idiotype (Id-460) during in vivo anti-dinitrophenyl antibody responses. I. Mapping of genes for Id-460 expression to the variable region of immunoglobulin heavy-chain locus and to the variable region of immunoglobulin kappa-light-chain locus [5].
• Nuclear extracts from pre-B and B cell lines contain a nuclear DNA binding protein (kappa locus protein, KLP) that specifically recognizes a DNA sequence in the immunoglobulin kappa light chain joining (J) segment gene region [6].
• Alternative splicing patterns in an aberrantly rearranged immunoglobulin kappa-light-chain gene [7].
• Cells of the mouse lymphocytoid pre-B-cell line 70Z/3 contain a constitutively rearranged immunoglobulin kappa light chain gene; the nucleotide sequence of this gene exhibits all the known properties of a functionally competent transcription unit [8].

Biological context of Igk-V28

• In the mouse, the genes coding for the Ly-2 antigen, the beta chain of the T-cell receptor, and the immunoglobulin kappa light chain have been located on chromosome 6 [9].
• A yeast artificial chromosome contig spanning the mouse immunoglobulin kappa light chain locus [10].

Anatomical context of Igk-V28

• We have used DNase I as a probe to examine the chromatin structure of mouse immunoglobulin kappa light chain genes in rearranged and unrearranged chromosomes--i.e., in nuclei from myeloma cells and from brain and liver cells [3].
• Immunoglobulin kappa light-chain diversity arises, in large part, from an array of germ-line V-region genes that undergo somatic recombination with one of four active J-region segments [11].

Other interactions of Igk-V28

• On the basis of these data, the Rn7s-6 sequence may be placed within 1.3 centimorgans of Ly-3 and one of the Igk-V-region markers, Igk-Ef1 [12].
• The results from the NAK mouse indicate that at least some members of Vk4, Vk8, Vk10, and Vk21 were on one side of the recombination event linked to the Lyt-2 alpha and Igk-Ef1 alpha alleles of AKR, while the Vk9, Vk11, and Vk24 REF patterns came from the NZB parental strain linked to the Igk-Ef2 beta (Vk1) allele [13].
• The mouse Igk-VSer gene encodes an immunoglobulin kappa light chain variable region which gives rise to two phenotypic polymorphisms of mouse kappa chains [14].
• Structural and evolutionary comparisons of four alleles of the mouse Igk-J locus which encodes immunoglobulin kappa light chain joining (J kappa) segments [15].

Analytical, diagnostic and therapeutic context of Igk-V28

• The limit of human IgG detection by inhibition ELISA using mouse anti-human immunoglobulin kappa light chain (Ig kappa) monoclonal antibody is 0.01 microgram [16].

References