Disease relevance of Fxyd3

• We recently identified a novel 8-kDa transmembrane protein, Mat-8, that is expressed in a subset of murine breast tumors [1].

High impact information on Fxyd3

• In this study, we have identified two splice variants of human FXYD3, or Mat-8, in CaCo-2 cells [2].
• Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes [1].
• It is a member of the FXYD family of single-span transmembrane proteins that include phospholemman, Mat-8, and the gamma-subunit of Na(+)-K(+)-ATPase [3].
• A Fxyd3-specific sense cRNA probe hybridized to a transcript in Northern blots of brain and colon RNA that co-migrated with the Lgi4 mRNA [4].
• Northern blot analyses demonstrated a 0.6-kb Fxyd3 transcript with abundant expression in the murine skin, colon, and mammary gland, but low level expression in the brain [4].

Anatomical context of Fxyd3

• In situ hybridization experiments revealed that both Fxyd3 and Lgi4 were expressed in the same tissue compartments in skin, uterus, intestine, mammary gland, and brain [4].
• We have now cloned a cDNA encoding the human version of Mat-8 and show that it is expressed both in primary human breast tumors and in human breast tumor cell lines [1].
• Molecular cloning and characterization of a novel phospholemman-like protein from rat hippocampus [5].
• The spherical structures observed were not colocalized with markers for lysosomes, endosomes, and Golgi bodies, suggesting that Mat-8 is distributed in a distinct endoplasmic reticulum region and the nuclear envelope after synthesis on membrane-bound ribosomes [6].

Associations of Fxyd3 with chemical compounds

• The Fxyd3 gene (formerly called Mat-8) encodes an 8-kDa transmembrane protein that is upregulated in mammary tumors and can induce a chloride conductance upon RNA injection into Xenopus oocytes [4].

Other interactions of Fxyd3

• Fxyd3 and Lgi4 expression in the adult mouse: a case of endogenous antisense expression [4].

Analytical, diagnostic and therapeutic context of Fxyd3

• We have investigated the expression of Fxyd3 and Lgi4 in the adult mouse by Northern blot analyses and in situ hybridization [4].

References