High impact information on mdh-1

• Proteins of young single Meloidogyne females were separated using polyacrylamide gradient gels and stained for the isozymes esterase and malate dehydrogenase [1].
• The intracellular distribution and high levels of activity observed for the enzymes phosphoenolpyruvate carboxykinase, pyruvate kinase, lactate dehydrogenase, malate dehydrogenase, malic enzyme and fumarate reductase suggest two alternative pathways of carbohydrate energy metabolism [2].
• Isozyme banding patterns of all the 8 enzymes were species specific; however, 3 enzymes, i.e., arginine kinase, fumarate hydratase, and malate dehydrogenase, displayed distinct patterns among all the 18 isolates [3].
• The presence of important enzymes of lipid biosynthesis, glucose-6-phosphate dehydrogenase, malate dehydrogenase and hydroxymethyl glutaryl-CoA reductase and an enzyme of lipid ester hydrolysis, triacylglycerol lipase, is also established in T. globulosa [4].
• Determinations were made of the in vitro inhibitory activities of four benzimidazoles and six synthesised pyrimidine derivatives on MDH (soluble and mitochondrial) from helminth parasites [5].

Associations of mdh-1 with chemical compounds

• Malate dehydrogenase in helminth parasites. Inhibition by benzimidazoles and pyrimidine derivatives [5].
• The presence of important enzymes of lipid biosynthesis like glucose-6-phosphate dehydrogenase, malate dehydrogenase and hydroxymethyl glutaryl-CoA reductase as well as an enzyme of lipid ester hydrolysis, triacylglycerol lipase is detected in the parasite [6].
• Haloxon had little effect on malate dehydrogenase activity of any of the parasite [7].
• Assay of malate dehydrogenase, following the in vitro drug treatment cambendazole and tiabendazole, exhibited moderate inhibition of the activity in both the parasites [7].

Analytical, diagnostic and therapeutic context of mdh-1

• Previous allozyme analyses demonstrated the existence of Teladorsagia circumcincta sheep and goat lines, based on the presence (goat line) or absence (sheep line) of an allele at the cathodic loci of malate dehydrogenase (MDH), with rapid migration (coded sR or super rapid) when starch gel electrophoresis was used [8].

References