High impact information on Pole

• The size of the pol epsilon transcript for the catalytic subunit (> 200 kDa) was investigated in both normal human fibroblasts and HeLa cells [1].
• No significant difference in the level of pol epsilon transcript in HeLa cells or fibroblasts was seen after ultraviolet irradiation [1].
• The purified pol epsilon preparation showed two polypeptides of > 200 and 55 kDa and a small amount of active 122-kDa proteolysis product on denaturing polyacrylamide gels [1].
• We have used cell extracts from Pol beta-deleted mouse fibroblasts to separate subfractions containing either Pol delta or Pol epsilon [2].
• Similar to the pol-epsilon catalytic subunits from other species, the pol-epsilon catalytic subunit contains domains for DNA polymerization and 3'-5' exonuclease in the N-terminal region, and two potential zinc-finger domains in the C-terminal regions [3].

Biological context of Pole

• Mouse DNA polymerase epsilon gene (Pole) maps to chromosome 5 [4].
• cDNA and structural organization of the gene Pole1 for the mouse DNA polymerase epsilon catalytic subunit [5].
• The level of pol epsilon activity tsFT20 was also significantly lower than in the parental cells, suggesting cooperation and/or interaction between pol alpha and epsilon, and some relevance of pol epsilon to DNA replication [6].

Other interactions of Pole

• Our data show for the first time that Pol delta and/or Pol epsilon are directly involved in the long-patch BER of abasic sites and might function as back-up system for Pol beta in one-gap filling reactions [2].

Analytical, diagnostic and therapeutic context of Pole

• Mouse polyclonal antiserum was produced to a 144-amino acid fragment of pol epsilon fused to staphylococcal protein A. This non-neutralizing polyclonal antiserum specifically recognized the catalytic subunit of pol epsilon by immunoblotting, but not that of pol alpha, beta, or delta [1].

References