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Gene Review

POL2  -  Pol2p

Saccharomyces cerevisiae S288c

Synonyms: DNA polymerase II subunit A, DNA polymerase epsilon catalytic subunit A, DUN2, N0825, YNL262W
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High impact information on POL2

  • Unlike sad1 mutants defective for multiple cell cycle checkpoints, pol2 mutants are defective only for the S phase checkpoint and the activation of DUN1 kinase necessary for the transcriptional response to damage [1].
  • We have cloned and analyzed POL2, the gene encoding the catalytic subunit of the third nuclear DNA polymerase, DNA polymerase II [2].
  • POL2 expressed a transcript of approximately 7.5 kb and contained a reading frame that encoded a protein of calculated Mr 255,649 [2].
  • These results show that POL2 and RAD9 function in parallel branches for sensing and transducing the UV DNA damage signal [3].
  • Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints [4].

Biological context of POL2


Associations of POL2 with chemical compounds


Other interactions of POL2

  • Repair synthesis in nuclear extracts of pol1, pol2, and pol3 temperature-sensitive mutants was normal at permissive temperatures [10].
  • We previously reported that MAG1 induction was abolished in pol2 and rad53 mutants, but not in the mec1-1 mutant [11].
  • The polymerase epsilon mutations, pol2-9 and pol2-18, which lead to temperature-sensitivity, and pol2-1 (insertion of URA3 at the position coding for amino acid 1134 in the POL2 gene) substantially reduced HAP mutagenesis [12].
  • Here we show that the epigenetic states of the telomere gene are not stably inherited in cells either bearing a mutation in a catalytic subunit (Pol2) of replicative DNA polymerase epsilon (Pol epsilon) or lacking one of the nonessential and histone fold motif-containing subunits of Pol epsilon, Dpb3 and Dpb4 [13].
  • We present evidence that pol2 mutants are defective in sister chromatid cohesion [14].


  1. DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint. Navas, T.A., Zhou, Z., Elledge, S.J. Cell (1995) [Pubmed]
  2. A third essential DNA polymerase in S. cerevisiae. Morrison, A., Araki, H., Clark, A.B., Hamatake, R.K., Sugino, A. Cell (1990) [Pubmed]
  3. RAD9 and DNA polymerase epsilon form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae. Navas, T.A., Sanchez, Y., Elledge, S.J. Genes Dev. (1996) [Pubmed]
  4. DNA polymerase epsilon catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. Kesti, T., Flick, K., Keränen, S., Syväoja, J.E., Wittenberg, C. Mol. Cell (1999) [Pubmed]
  5. DRC1, DNA replication and checkpoint protein 1, functions with DPB11 to control DNA replication and the S-phase checkpoint in Saccharomyces cerevisiae. Wang, H., Elledge, S.J. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  6. Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Araki, H., Leem, S.H., Phongdara, A., Sugino, A. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  7. Role of the putative zinc finger domain of Saccharomyces cerevisiae DNA polymerase epsilon in DNA replication and the S/M checkpoint pathway. Dua, R., Levy, D.L., Campbell, J.L. J. Biol. Chem. (1998) [Pubmed]
  8. Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae. Araki, H., Hamatake, R.K., Morrison, A., Johnson, A.L., Johnston, L.H., Sugino, A. Nucleic Acids Res. (1991) [Pubmed]
  9. In vivo reconstitution of Saccharomyces cerevisiae DNA polymerase epsilon in insect cells. Purification and characterization. Dua, R., Levy, D.L., Li, C.M., Snow, P.M., Campbell, J.L. J. Biol. Chem. (2002) [Pubmed]
  10. DNA repair synthesis during base excision repair in vitro is catalyzed by DNA polymerase epsilon and is influenced by DNA polymerases alpha and delta in Saccharomyces cerevisiae. Wang, Z., Wu, X., Friedberg, E.C. Mol. Cell. Biol. (1993) [Pubmed]
  11. Two alternative cell cycle checkpoint pathways differentially control DNA damage-dependent induction of MAG1 and DDI1 expression in yeast. Zhu, Y., Xiao, W. Mol. Genet. Genomics (2001) [Pubmed]
  12. Base analog 6-N-hydroxylaminopurine mutagenesis in the yeast Saccharomyces cerevisiae is controlled by replicative DNA polymerases. Shcherbakova, P.V., Noskov, V.N., Pshenichnov, M.R., Pavlov, Y.I. Mutat. Res. (1996) [Pubmed]
  13. Noncompetitive counteractions of DNA polymerase epsilon and ISW2/yCHRAC for epigenetic inheritance of telomere position effect in Saccharomyces cerevisiae. Iida, T., Araki, H. Mol. Cell. Biol. (2004) [Pubmed]
  14. Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion. Edwards, S., Li, C.M., Levy, D.L., Brown, J., Snow, P.M., Campbell, J.L. Mol. Cell. Biol. (2003) [Pubmed]
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