Disease relevance of NR2F6

• Recombinant Ear2 was purified from Escherichia coli and an antipeptide antisera was generated [1].
• CONCLUSIONS: These findings suggest that rat EAR-1 and EAR-2 act as host defense factors against bacterial infection in rats [2].

High impact information on NR2F6

• The Sp1(I) site was identified as critical for the repression since its mutation reduced the inhibition by EAR2 and abolished the inhibition by EAR3 [3].
• The orphan nuclear receptor Ear-2 is a negative coregulator for thyroid hormone nuclear receptor function [4].
• A double mutation in elements CIIB and CIIC that eliminated binding of HNF-4 or ARP-1 and EAR-2, respectively, to these sites abolished the enhancer activity of HCR-1 [5].
• In addition, hCG treatment significantly reduced the inhibition of rLHR gene in granulosa cells and also decreased EAR2 and EAR3/COUP-TFI protein levels [6].
• Cloning, expression and regulation of chicken ovalbumin upstream promoter transcription factors (COUP-TFII and EAR-2) in the rat anterior pituitary gland [7].

Biological context of NR2F6

• The stimulation was reversed by coexpression of EAR2 or EAR3/COUP-TFI, indicating their competitive binding for this site [8].
• Cotransfection experiments in HepG2 cells revealed that ARP-1, EAR-2, and EAR-3 repressed the BA1, CIIIB, and AIIJ element-dependent transcription of the reporter gene constructs and the transcription driven by homopolymeric promoters containing either five BA1 or two CIIIB elements [9].
• To better understand their role in the differentiated adult rat pituitary gland, we cloned COUP-TFII and EAR-2 cDNAs from an anterior pituitary cDNA library [7].
• Our results suggest that in normal breast tissue, the function of promoters I.3 and II is suppressed through the binding of EAR-2, COUP-TFI, and RARgamma to S1, and through the binding of Snail/Slug proteins to their binding site that quenches the CREaro activity [10].
• EMSA showed that purified recombinant Ear2 specifically binds the TGACCT direct-repeat motif [1].

Associations of NR2F6 with chemical compounds

• Transfection assays showed that Ear2 potently decreases both baseline and retinoid-induced mouse renin promoter activity in a dose-dependent, enhancer-dependent, and sequence-specific manner [1].

Other interactions of NR2F6

• Identification of a nuclear orphan receptor (Ear2) as a negative regulator of renin gene transcription [1].
• METHODS: As we have already cloned the complete nucleotide sequence for rat EAR-1, we determined that for rat EAR-2 cDNA by the rapid amplification of cDNA ends procedure [2].

References