Disease relevance of LOC396058

• There is strong correlation between hypersensitivity at regions I-IV and gene transcription; ovalbumin mRNA levels are high in stimulated chicks, where hypersensitive regions are present, and drop to very low levels in withdrawn chicks, where the hypersensitivity is absent [1].
• The bacterial synthesis of significant amounts of chicken ovalbumin demonstrates that the E. coli cellular machinery may be utilized to synthesize a higher eukaryotic protein which is relatively stable in the bacterial intracellular environment [2].
• The entire chicken ovalbumin gene, accompanied by genomic DNA sequences flanking both termini of the gene and three copies of the herpes simplex virus thymidine kinase gene, has been cloned in plasmid pBR322 [3].
• We show that the COUP (chicken ovalbumin upstream promoter) orphan receptors also bind to the HIV-1 RARE and repress the retinoid response of the HIV-1 RARE or the HIV-1 LTR [4].
• The principle of delivery of T cell epitopes to antigen-presenting cells applied to peptides from influenza virus, ovalbumin, and hen egg lysozyme: implications for peptide vaccination [5].

Psychiatry related information on LOC396058

• Ovalbumin subfractionation and individual difference in ovalbumin microheterogeneity [6].
• In the ovalbumin control locus up to the 6 kb upstream region, CpG sites were methylated in immature chickens, except for several sites, and almost all CpGs residing in DNase I hypersensitive sites I, II, and III, but not IV, were selectively unmethylated in ovalbumin expressing chickens [7].

High impact information on LOC396058

• Four TGACC half-palindromic motifs, separated from each other by more than 100 bp, are responsible for conferring estrogen inducibility either to the proximal ovalbumin gene promoter or to heterologous promoters [8].
• The ovalbumin promoter half-palindromic ERE motif located close to the TATA box was required for the activity of the distal DH3 ERE, but could be replaced by the binding sites of other transactivators [8].
• A far upstream estrogen response element of the ovalbumin gene contains several half-palindromic 5'-TGACC-3' motifs acting synergistically [8].
• We have identified an estrogen-responsive enhancer element (DH3 ERE) in the estrogen-induced DNAase I-hypersensitive region III of the chicken ovalbumin gene, which is located approximately 3.3 kb upstream from the mRNA start site and does not contain palindromic ERE [8].
• The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells [9].

Chemical compound and disease context of LOC396058

• The correlation of hypersensitivity with transcription was determined for each region in oviducts of chicks, where expression of the ovalbumin gene can be controlled by administration and withdrawal of steroid hormones [1].
• When a plasmid containing the hybrid gene was introduced into E. coli, a protein identified as ovalbumin by immunoreactivity and sodium dodecyl sulfate/polyacrylamide gel electrophoresis was synthesized [2].
• Both Ia-negative B lymphoma cells and mouse L cells were capable of antigen presentation of predigested ovalbumin after fusion with vesicles formed from phosphatidylserine and phosphatidylethanolamine in a 1:1 w:w ratio [10].
• An ovalbumin complementary DNA clone was manipulated in vitro, and constructs containing altered protein-coding sequences and either the simian virus 40 (SV40) early promoter or Herpes simplex thymidine kinase promoter, were microinjected into Xenopus laevis oocytes [11].
• Synthesis of ovalbumin in fragmented oviduct magnum explants of immature, estrogen-stimulated chicks has been studied in the presence of exogenous tRNA. tRAN from Novikoff hepatoma specifically inhibited ovalbumin synthesis, determined by precipitation with antisera [12].

Biological context of LOC396058

• At least one of these Hae III sites is situated in the intervening sequence of the ovalbumin gene, and its lcoation has been mapped [13].
• Nucleotide sequence homology at 12 intron--exon junctions in the chick ovalbumin gene [14].
• A short partial sequence homology is present at all intron-exon junctions, or splice points, in the chick ovalbumin gene; it is probably a signal for a splicing enzyme [14].
• The sequence of 35 residues at the NH2 terminus of ovalbumin was determined by automated Edman degradation after a method was devised to prevent acetylation during protein synthesis in the reticulocyte lysate [15].
• The steroid-dependent regulatory element in the ovalbumin gene does not function as a typical steroid response element [16].

Anatomical context of LOC396058

• Ovalbumin mRNA was translated in a reticulocyte lysate [15].
• Results of these experiments revealed that 50 to 60 amino acid residues are sufficient to bind ovalbumin-synthesizing polysomes to membranes in vitro [17].
• Kinetic experiments were performed in a cell-free translation system to measure the minimum size of ovalbumin nascent chains required for binding of both the nascent chain and the corresponding mRNA to microsomal membranes derived from dog pancreas [17].
• The observation that the transcriptionally active ovalbumin gene is preferentially associated with the nuclear matrix may have significant implications for gene expression and the organization of nuclear DNA into supercoiled-loop domains [9].
• Expression of a chicken chromosomal ovalbumin gene injected into frog oocyte nuclei [18].

Associations of LOC396058 with chemical compounds

• These data indicate that induction of the ovalbumin gene by steroid hormones requires complex interactions involving both the SDRE and the negative regulatory element [16].
• To characterize the regulatory properties of the SDRE more precisely, additional mutations were created in this region, and fusion genes prepared by linking the ovalbumin 5'-flanking region and promoter to the chloramphenicol acetyltransferase structural gene [16].
• In contrast, I-ovalbumin, the product of a heating transition, is a potent reversible serine proteinase inhibitor [19].
• We conclude that ovalbumin Y is a unique chimeric glycoprotein having an amino acid sequence similar to that of ovalbumin, but having a carbohydrate moiety similar to that of ovomucoid [20].
• The overlap of the C-terminal sequence of this extended sequence with the six-residue N-terminal sequence surrounding a half-cystine residue in ovalbumin gives the N-terminal sequence for ovalbumin as acetyl-Gly-Ser-Ile-Gly-Ala-Ala-Ser-Met-Glu-Phe-Cys-Phe-Asp-Val-Phe-Lys with residue 11 a cysteine residue [21].

Physical interactions of LOC396058

• We suggest that the interaction of a somatomedin with its membrane-bound receptor generates an intracellular signal that interacts specifically with the ovalbumin gene [22].
• The purified DNA binding component (receptor A) of the chick oviduct progesterone receptor has been analyzed for its ability to bind to the cloned ovalbumin gene and to plasmid DNA of various structural compositions [23].
• By means of the DNA-cellulose competitive binding assay, the interaction of estrogen receptor complexed to 17 beta-estradiol with fragments of a cloned DNA region of the estrogen responsive chicken ovalbumin gene spanning from 1343 bps upstream to 373 bps within the transcribed region of the gene (p0V 1.7) was investigated [24].
• Disposable polypropylene plates were coated with specific IgG antibody and used for radioimmunoassay with 125I-specific IgG antibody to aldolase A. The non-specific binding was minimized by saturating the binding sites of the plates with 2% ovalbumin in 0.1% Tween 20 [25].
• The results indicated that neither the NF1 binding element nor the ovalbumin upstream promoter showed any enhancer-like activity [26].

Enzymatic interactions of LOC396058

• Rather, an analysis of the fragmentation of the ovalbumin chromatin as a function of digestion extent suggested a mechanism in which the heightened sensitivity resulted from the collective expansion of the nuclease cutting sites in the linker regions of the ovalbumin chromatin because the gene was in an unfolded conformation [27].

Regulatory relationships of LOC396058

• Peroxisome proliferator-activated receptor gamma and chicken ovalbumin upstream promoter transcription factor II negatively regulate the phosphoenolpyruvate carboxykinase promoter via a common element [28].
• Fluorescently labeled ovalbumin and bovine serum albumin were also injected as control proteins. alpha-Actinin was incorporated into stress fibers within 5 minutes after injection and remained present in the fibers for up to 11 days [29].
• Estrogen receptor gene transfection induced ovalbumin mRNA to a moderate extent in the absence of the steroid hormones [30].
• In this study, procaterol dose-dependently inhibited IFN-gamma production of peripheral blood mononuclear cells stimulated with ovalbumin in patients with hen's egg-sensitive atopic dermatitis, without inhibition of proliferative responses of peripheral blood mononuclear cells [31].

Other interactions of LOC396058

• Ovalbumin, the major protein in avian egg-white, is a non-inhibitory member of the serine protease inhibitor (serpin) superfamily [32].
• The sequence was related to members of the serpin gene family, particularly ovalbumin and the chicken gene Y protein [33].
• Commercially purified ovomucoid resulted in only minimal amounts of antibodies to ovalbumin [34].
• Ovomucoid mean wheal diameters were significantly greater than wheal diameters in response to ovalbumin, lysozyme, and egg white extract at the three most concentrated of five dilutions tested: 0.01, 0.03, and 0.1 mg/ml (p < 0.01) [34].
• A known estrogen-inducible protein, ovalbumin, was localized in the same glandular epithelial cells as ER [35].

Analytical, diagnostic and therapeutic context of LOC396058

• Electron microscopy and restriction enzyme mapping reveal additional intervening sequences in the chicken ovalbumin split gene [36].
• These results are consistent with transcription of the entire ovalbumin gene into a large precursor molecule followed by excision of the intervening sequences and appropriate ligation of the structural sequences to form the mature mRNA [37].
• Two large DNA fragments overlapping the chicken ovalbumin gene have been isolated by molecular cloning [38].
• In order to prove that a monoclonal antibody, KJ1-26.1, reacted with the idiotypic portion of the receptor for antigen plus major histocompatibility complex product (Ag/MHC) on the T cell hybridoma D0-11.10, 397 independent T cell hybridomas prepared from BALB/c T cells primed with ovalbumin were screened by ELISA for reactivity with the antibody [39].
• Steroid hormone regulation of activity of the chicken ovalbumin promoter was studied by microinjection of chimeric genes into the nuclei of primary cultured oviduct tubular gland cells [40].

References