High impact information on Alg12

• Congenital disorders of glycosylation type Ig is defined by a deficiency in dolichyl-P-mannose:Man7GlcNAc2-PP-dolichyl mannosyltransferase [1].
• We conclude that the same mannosyltransferase responsible for dolichyl phosphate mannose synthesis can also utilize in vitro exogenous retinyl phosphate and phosphatidic acid as well as endogenous phosphatidic acid as mannosyl acceptors [2].
• The activity of GDPmannose:dolichyl monophosphate mannosyltransferase in inner mitochondrial membranes can be triggered by dolichyl-monophosphate incorporation mediated through phospholipids or fatty acids [3].
• Three glycosyltransferases, namely N-acetylglucosaminyl-1-phosphotransferase, mannosyltransferase and glucosyltransferase, involved in the biosynthesis of asparagine-linked glycoproteins in the mouse mammary gland, were identified by characterization of the products formed by these enzymes [4].
• Glycosyltransferase activities in liver mitochondria. Phospholipid-dependence of inner membrane mannosyltransferase [5].

Anatomical context of Alg12

• Purification and properties of a mannosyltransferase solubilized from mitochondrial outer membranes [6].
• Previous studies have shown the existence of an autonomous mitochondrial GDPmannose:dolichylmonophosphate mannosyltransferase, located in mitochondrial outer membrane of liver cells [7].

Associations of Alg12 with chemical compounds

• Addition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and cardiolipin in the presence of Mg2+ results in a high degree of activation of the mannosyltransferase system [5].
• Topological orientation of mitochondrial GDPmannose: dolichyl-monophosphate mannosyltransferase in the outer membrane [7].
• The partially purified mannosyltransferase is activated by choline-containing lipids such as phosphatidylcholine, lysophosphatidylcholine and sphingomyelin [6].

References