Disease relevance of Retinyl phosphate

• Retinyl palmitate, retinyl phosphate, and dolichyl phosphate of postnuclear membrane fraction from hepatoma, host liver, and regenerating liver: marginal vitamin A status of hepatoma tissue [1].
• Class E Thy-1-negative mutant mouse lymphoma cell membranes, which are defective in dolichyl phosphate mannose synthesis, also fail to transfer mannose from GDP-mannose to exogenous phosphatidic acid or retinyl phosphate [2].

High impact information on Retinyl phosphate

• This mannolipid was believed to be synthesized from endogenous retinyl phosphate and was tentatively identified as retinyl phosphate mannose (Ret-P-Man) (De Luca, L. M., Brugh, M. R. Silverman-Jones, C. S. and Shidoji, Y. (1982) Biochem. J. 208, 159-170) [2].
• Rat liver membranes did not catalyze the transfer of [14C]galactose from UDP-[14C]galactose to retinyl phosphate even at a concentration of retinyl phosphate (0.73 mM) which stimulated formation o mannosylretinyl phosphate by more than 50-fold.. [3].
• The anomeric configuration of the D-mannosyl retinyl phosphate formed in rat liver microsomes [4].
• No evidence for enzymatic hydrolysis of D-mannosyl retinyl phosphate by alpha-mannosidase was obtainable [4].
• Mannosyl carrier functions of retinyl phosphate and dolichyl phosphate in rat liver endoplasmic reticulum [5].

Biological context of Retinyl phosphate

• Thus, hydrogenation of side-chain double bonds in retinyl phosphate impaired only slightly its mannosyl acceptor activity, but caused loss of mannosyl donor activity [6].
• Presence of a cellular retinylphosphate binding protein in rat liver [7].

Anatomical context of Retinyl phosphate

• The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver [8].
• Isolation, characterization, and biological activity of retinyl phosphate from hamster intestinal epithelium [9].
• A retinylphosphate binding activity, resolved during purification, has been discovered in rat liver cytosol [7].

Associations of Retinyl phosphate with other chemical compounds

• Of the subcellular fractions of rat liver the endoplasmic reticulum was the most active in GDP-mannose: retinyl phosphate mannosyl-transfer activity [5].
• In the presence of Mn(II) ions, the u.v. absorption spectrum of retinyl phosphate (Ret-P) solubilized in Triton X-100 micelles, phosphatidylcholine liposomes or rat liver microsomes exhibited a shift from the maximum of 330 nm to 287 nm [10].
• Recent studies on the involvement of retinyl phosphate as a carrier of mannose in biological membranes [11].

Gene context of Retinyl phosphate

• Endoplasmic reticulum, but not Golgi-apparatus membranes, catalysed significant transfer of [14C]mannose to endogenous acceptor proteins in the presence of exogenous retinyl phosphate [5].

References