Disease relevance of MAPRE3

• In pull-down assays VP26+ capsids bound to RP3; VP26-capsids did not [1].
• Herpes simplex virus type 1 capsid protein VP26 interacts with dynein light chains RP3 and Tctex1 and plays a role in retrograde cellular transport [1].
• One locus (RP3) has been closely defined by genetic linkage and deletion analyses and localised to the region between the ornithine transcarbamylase (OTC) and chronic granulomatous disease (CYBB) loci in Xp21.1-p11 [2].
• PURPOSE: To describe fundus autofluorescence (AF) in carriers of X-linked retinitis pigmentosa (XLRP) associated with mutations in RPGR (RP3), and to compare the findings on AF with ophthalmoscopy and with electrophysiological and psychophysical data [3].
• Cell lines containing EBV in the productive state (EB3 and P3HR1) were positive with ISH for NotI, while a latently infected cell line (Raji) was negative [4].

High impact information on MAPRE3

• Since the R component, but not VCA, was found to be denatured by methanol fixation, the parallel use of acetone- and methanol-fixed EB3 cell smears permitted the determination of the correct anti-VCA titer [5].
• African patients with Burkitt's lymphoma (BL) may show antibodies to the R (restricted) component of the Epstein-Barr (EB) virus-induced early antigens that appear to match in titer the antibodies to EB viral capsid antigen (VCA) as determined with the aid of acetone-fixed smears of EB3 cells [5].
• The RPGR (retinitis pigmentosa GTPase regulator) gene for RP3, the most frequent genetic subtype of X-linked retinitis pigmentosa (XLRP), has been shown to be mutated in 10%-15% of European XLRP patients [6].
• Moreover, in these experiments EB3 and APCL appeared together in the perinucleus and the cytoplasmic microtubule network [7].
• EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue [7].

Anatomical context of MAPRE3

• Two human lymphoblasts (Raji and EB3) and normal human peripheral lymphocytes were exposed to different concentrations of Concanavalin A and wheat germ agglutinin [8].
• Not all human amnions yielded cells transformable by EB3 cell sonicate, as determined by direct comparisions using the same cultural conditions and testing with the same fresh sonicate preparation in the same experiment [9].

Other interactions of MAPRE3

• This deletion has now been shown to disrupt the RP3 (RPGR) gene [10].
• The YAC contains the CYBB locus in Xp21.1 and is thought to contain at least part of the RP3 gene responsible for X-linked retinitis pigmentosa [11].
• Immunostaining also revealed that APC and p150glued were present in EB1-DeltaN2-GFP aggregates, whereas EB3 was not [12].
• So far, diagnostic services have been offered only to those families in which linkage to one RP locus (RP2 or RP3) has been clearly established [13].

Analytical, diagnostic and therapeutic context of MAPRE3

• Using the SDS-polyacrylamide immunoblotting technique, it was shown that one epitope (shared with human chorionic somatomammotropin) detected by EB1 (or EB3) antibody was expressed to a similar extent by both the N-terminal (15 K) and C-terminal (7 K) polypeptides [14].

References