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Gene Review:

RT1-EC2 - RT1 class Ib, locus EC2

Rattus norvegicus

Synonyms: Class I histocompatibility antigen, Non-RT1.A alpha-1 chain, MHCRTA, RATMHCRTA, RT1-A, RT1-Aw2, ...

Kraus, E. et al., González-Muñoz, A.L. et al., Hoek, K.S. et al., Chueh, S.C. et al., Okamoto, M. et al., et al.

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Disease relevance of RT1-Aw2

Transfection of RT1.Al protected both L-929 fibroblasts and P815 mastocytoma cells from lysis by the NK cell line, while RT1.Cl only protected P815 cells, indicating that additional target cell properties regulate rat NK cell activity [1].

High impact information on RT1-Aw2

RT1-A1(o/d) and -A2(o/d) are closely related in sequence to other cloned rat class Ia genes that have been shown to map to the RT1-A region, while RT1-A3 degrees is highly homologous to a class I gene identified by sequencing an RT1-A(n) genomic contig and is named A3(n) [2].
A novel instance of class I modification (cim) affecting two of three rat class I RT1-A molecules within one MHC haplotype [2].
The class Ia molecules are encoded by the RT1-A region of the rat MHC, while the RT1-C/E/M region encodes up to 60 other class I genes or gene fragments, a number of which are known to be expressed (or to be expressible) [3].
A group of six 35-42-kDa proteins bind to a 35-base oligoribonucleotide incorporating the RTS, but not to several oligoribonucleotides with the same composition but randomized sequences, thus establishing specificity for the base sequence in the RTS [4].

Biological context of RT1-Aw2

METHODS: Allochimeric alpha(1h)l58-80-RT1.Aa proteins were produced by substituting the allogeneic nucleotide sequence encoding 10 aa residues unique to the alpha1 helical (alpha1h) region of RT1.Al Lewis (Asp58, Arg62, Glu63, Gln65, Lys66, Gly69, Asn70, Asn73, Ser77, and Asn80) for native RT1.Aa residues [5].

Anatomical context of RT1-Aw2

This was demonstrated with mouse-rat hybridoma target cells expressing different rat MHC alleles and with mouse tumor target cells transfected with classical (RT1.Al) and nonclassical (RT1.Cl) rat MHC class I genes [1].
In order to do this, Lewis rats which carry major histocompatibility complex (MHC) class I antigens haplotype 1 (RT1.Al) were reconstituted with (Lew X PVG)F1 (RT1.Al/c) bone marrow cells after lethal irradiation [6].

Analytical, diagnostic and therapeutic context of RT1-Aw2

Sequence analysis of the promoter regions of the classical class I gene RT1.Al and two other class I genes of the rat MHC [7].
It was separated from the other polypeptides by using reverse-phase HPLC and shown to retain preferential association with the RTS [4].
In the GvHD model, ACI (RT1a) donors were pretreated (day -14) with an intrathymic injection of alpha(1h)l58-80-RT1.Aa, alpha(1h)l/u62-69-RT1.Aa, or RT1.Al protein, with or without simultaneous intravenous injection of anti-T-cell receptor R73 monoclonal antibodies [8].

References

Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Kraus, E., Lambracht, D., Wonigeit, K., Hünig, T. Eur. J. Immunol. (1996) [Pubmed]
A novel instance of class I modification (cim) affecting two of three rat class I RT1-A molecules within one MHC haplotype. González-Muñoz, A.L., Le Rolle, A.F., Brun, H., Hedrich, H.J., Wedekind, D., Powis, S.J., Joly, E., Butcher, G.W. J. Immunol. (2003) [Pubmed]
RT1-U: identification of a novel, active, class Ib alloantigen of the rat MHC. Leong, L.Y., Le Rolle, A.F., Deverson, E.V., Powis, S.J., Larkins, A.P., Vaage, J.T., Stokland, A., Lambracht-Washington, D., Rolstad, B., Joly, E., Butcher, G.W. J. Immunol. (1999) [Pubmed]
hnRNP A2 selectively binds the cytoplasmic transport sequence of myelin basic protein mRNA. Hoek, K.S., Kidd, G.J., Carson, J.H., Smith, R. Biochemistry (1998) [Pubmed]
Induction of tolerance toward rat cardiac allografts by treatment with allochimeric class I MHC antigen and FTY720. Chueh, S.C., Tian, L., Wang, M., Wang, M.E., Stepkowski, S.M., Kahan, B.D. Transplantation (1997) [Pubmed]
Absence of donor-type major histocompatibility complex class I antigen-bearing microglia in the rat central nervous system of radiation bone marrow chimeras. Matsumoto, Y., Fujiwara, M. J. Neuroimmunol. (1987) [Pubmed]
Sequence analysis of the promoter regions of the classical class I gene RT1.Al and two other class I genes of the rat MHC. Lambracht, D., Wonigeit, K. Immunogenetics (1995) [Pubmed]
Use of allochimeric proteins to mitigate graft-versus-host and host-versus-graft immune responses to rat small bowel allografts. Okamoto, M., Stepkowski, S.M., Wang, M., Kahan, B.D. Transplantation (2000) [Pubmed]

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