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Gene Review

tetR  -  hypothetical protein

Escherichia coli

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Disease relevance of tetR

  • TetR repressor proteins from the Agrobacterium tet system and from RP4 interacted with the heterologous operators [1].
  • The fates of the chromosome ori and ter loci were monitored by visualization by using derivatives of LacI and TetR fused to fluorescent proteins in E. coli strains that carry operator arrays at the appropriate locations [2].
  • We used E. coli strains with either TetR or the reverse variant revTetR(r2), while only TetR was employed in Salmonella [3].

High impact information on tetR

  • There is, in addition, significant amino acid sequence homology between an NH2-terminal region of the Tn10 TetR repressor and the DNA recognition regions of other DNA-binding proteins [4].
  • Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA) [5].
  • Tigecycline induced the Tet(B) protein approximately four times more efficiently than tetracycline, as determined by Western blotting, indicating that it is at least recognized by a TetR repressor [6].
  • Expression of beta-galactosidase by the fusion plasmid pUB3610 remained subject to regulatory control by the TetR repressor protein, with the presence of tetracyclines in the growth medium leading to a 12-fold induction of beta-galactosidase synthesis [7].
  • It randomly fuses the TetR-inducing peptide Tip to the affected reading frame [8].

Biological context of tetR

  • It contains three 38-bp inverted repeats, and (in this order) a new orfI, a resolution site (res), genes encoding resolvase (tnpR), transposase (tnpA), tetracycline-resistance (TcR) repressor (tetR), TcR (tetA) and a truncated transposase gene (tnpA') [9].
  • There is 47% amino acid sequence homology between the deduced sequences of the Tn10 and RP1/Tn1721 TetR proteins [4].
  • Contrary to dimeric TetR, scTetR allows the construction of scTetR mutants, in which one subunit contains a defective inducer binding site while the other is functional [10].
  • Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression [8].


  1. Cloning and characterization of a tetracycline resistance determinant present in Agrobacterium tumefaciens C58. Luo, Z.Q., Farrand, S.K. J. Bacteriol. (1999) [Pubmed]
  2. Sublethal Concentrations of the Aminoglycoside Amikacin Interfere with Cell Division without Affecting Chromosome Dynamics. Possoz, C., Newmark, J., Sorto, N., Sherratt, D.J., Tolmasky, M.E. Antimicrob. Agents Chemother. (2007) [Pubmed]
  3. Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase. Köstner, M., Schmidt, B., Bertram, R., Hillen, W. Appl. Environ. Microbiol. (2006) [Pubmed]
  4. Nucleotide sequence of the repressor gene of the TN10 tetracycline resistance determinant. Postle, K., Nguyen, T.T., Bertrand, K.P. Nucleic Acids Res. (1984) [Pubmed]
  5. The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis. Waters, S.H., Rogowsky, P., Grinsted, J., Altenbuchner, J., Schmitt, R. Nucleic Acids Res. (1983) [Pubmed]
  6. Effects of efflux transporter genes on susceptibility of Escherichia coli to tigecycline (GAR-936). Hirata, T., Saito, A., Nishino, K., Tamura, N., Yamaguchi, A. Antimicrob. Agents Chemother. (2004) [Pubmed]
  7. Sensitive biological detection method for tetracyclines using a tetA-lacZ fusion system. Chopra, I., Hacker, K., Misulovin, Z., Rothstein, D.M. Antimicrob. Agents Chemother. (1990) [Pubmed]
  8. Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression. Schlicht, M., Berens, C., Daam, J., Hillen, W. Appl. Environ. Microbiol. (2006) [Pubmed]
  9. Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein. Allmeier, H., Cresnar, B., Greck, M., Schmitt, R. Gene (1992) [Pubmed]
  10. Induction of single chain tetracycline repressor requires the binding of two inducers. Kamionka, A., Majewski, M., Roth, K., Bertram, R., Kraft, C., Hillen, W. Nucleic Acids Res. (2006) [Pubmed]
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