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Gene Review

X  -  p10

Borna disease virus

 
 
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Disease relevance of X

 

High impact information on X

  • Characterization of an unusual importin alpha binding motif in the borna disease virus p10 protein that directs nuclear import [3].
  • These findings suggest that nuclear import of the Borna disease virus p10 protein occurs through a nonconventional karyophilic signal and highlight that the cellular importin alpha NLS receptor proteins can recognize nuclear targeting signals that substantially deviate from the consensus sequence [3].
  • Of the six open reading frames of BDV, CTLs to BDV X (p10) and the L-polymerase have never been studied [1].
  • Three injections of a recombinant eukaryotic expression plasmid of BDV p10 were needed to generate a weak anti-p10 immunoglobulin M response [1].
  • Hence, p10 may play a role in the replication of BDV in the cell nucleus [4].
 

Biological context of X

  • Furthermore, binding of p10 to the viral phosphoprotein was shown to be important for association with the BDV-specific intranuclear clusters that may represent the sites of virus replication and transcription in infected cells [5].
  • In this study, we cloned the nucleotide sequence of ORFx1 into expression vectors and showed that it is expressed as p10 [6].
  • Four regions of the BDV genome were analysed: the complete p40, p10 and p24 genes and the 5'-untranslated region of the X/P transcript [7].
 

Analytical, diagnostic and therapeutic context of X

  • The time course for appearance of antibodies to Borna disease virus (BDV) major antigens, p40, p24, p18 and p10 were investigated in BDV-inoculated adult rats by Western blotting [8].
  • The successful isolation of the corresponding mRNA from infected cells, amplification of the genetic region by RT-PCR and its efficient expression as a glutathione S-transferase (GST) fusion protein demonstrated that antibodies specific for the BDV p10 protein are induced in infected animals [9].

References

  1. Two major histocompatibility complex class I-restricted epitopes of the Borna disease virus p10 protein identified by cytotoxic T lymphocytes induced by DNA-based immunization. Hashimoto, Y., Chen, H.S., Cunningham, C., Malik, T.H., Lai, P.K. J. Virol. (2003) [Pubmed]
  2. Prevalence of circulating antibodies to p10, a non-structural protein of the Borna disease virus in cats with ataxia. Ouchi, A., Kishi, M., Kobayashi, T., Lai, P.K., Malik, T.H., Ikuta, K., Mochizuki, M. J. Vet. Med. Sci. (2001) [Pubmed]
  3. Characterization of an unusual importin alpha binding motif in the borna disease virus p10 protein that directs nuclear import. Wolff, T., Unterstab, G., Heins, G., Richt, J.A., Kann, M. J. Biol. Chem. (2002) [Pubmed]
  4. Characterization of the P protein-binding domain on the 10-kilodalton protein of Borna disease virus. Malik, T.H., Kishi, M., Lai, P.K. J. Virol. (2000) [Pubmed]
  5. A short leucine-rich sequence in the Borna disease virus p10 protein mediates association with the viral phospho- and nucleoproteins. Wolff, T., Pfleger, R., Wehner, T., Reinhardt, J., Richt, J.A. J. Gen. Virol. (2000) [Pubmed]
  6. Nuclear localization of the protein from the open reading frame x1 of the Borna disease virus was through interactions with the viral nucleoprotein. Malik, T.H., Kobayashi, T., Ghosh, M., Kishi, M., Lai, P.K. Virology (1999) [Pubmed]
  7. Genetic clustering of Borna disease virus natural animal isolates, laboratory and vaccine strains strongly reflects their regional geographical origin. Kolodziejek, J., Dürrwald, R., Herzog, S., Ehrensperger, F., Lussy, H., Nowotny, N. J. Gen. Virol. (2005) [Pubmed]
  8. Antibodies to Borna disease virus in infected adult rats: an early appearance of anti-p10 antibody and recognition of novel virus-specific proteins in infected animal brain cells. Watanabe, M., Kobayashi, T., Tomonaga, K., Ikuta, K. J. Vet. Med. Sci. (2000) [Pubmed]
  9. Detection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues. Wehner, T., Ruppert, A., Herden, C., Frese, K., Becht, H., Richt, J.A. J. Gen. Virol. (1997) [Pubmed]
 
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