Disease relevance of DPYD

• Purification and characterization of dihydropyrimidine dehydrogenase from Alcaligenes eutrophus [1].

High impact information on DPYD

• Dihydropyrimidine dehydrogenase (DPDase) catalyzed the debromination of 5-bromo-5,6-dihydrouracil (BrUH2) to uracil at pH 7.7 and 37 degrees C. The debrominating activity of DPDase was increased 5-fold by treatment with H2O2, whereas the dehydrogenating activity was inhibited by this treatment [2].
• Dehalogenating and NADPH-modifying activities of dihydropyrimidine dehydrogenase [2].
• Inactivation of dihydropyrimidine dehydrogenase by 5-iodouracil [3].
• Since only 18% of [6-3H]5-iodouracil reduced by DHPDHase was covalently bound to the enzyme and radiolabel was not lost to the solvent as tritium, the partition coefficient for inactivation was 4 [3].
• DHPDHase was inactivated by 2.1 mol of racemic 5-iodo-5,6-dihydrouracil per mol of active sites [3].

Chemical compound and disease context of DPYD

• Dihydropyrimidine dehydrogenase from Alcaligenes eutrophus was purified to homogeneity using ammonium sulfate fractionation and chromatography on phenyl-Sepharose, MonoQ-Sepharose, and 2,5-ADP-Sepharose [1].

Biological context of DPYD

• Comparison of the nucleotide and deduced amino acid sequences of Bovine DPD to Pig and Human liver DPD reveals 93% and 92% identity respectively [4].
• Known amino acid sequence from purified bovine DPD was used to initially design mixed oligonucleotide primers for amplification of a cDNA fragment (65 base pairs) [4].

Associations of DPYD with chemical compounds

• Dihydropyrimidine dehydrogenase (DPD), the initial and rate-limiting enzyme in pyrimidine catabolism, has recently been purified to homogeneity from several species [4].
• Dihydropyrimidine dehydrogenase. Kinetic mechanism for reduction of uracil by NADPH [5].
• Dihydropyrimidine dehydrogenase catalyzes the rate-limiting step in the degradation of pyrimidines in mammals, the reduction of uracil or thymine to their 5,6-dihydro derivatives [6].
• Incubation of bone specimens at 37 degrees C for 60 days increased the level (per molecule of collagen) of PYD (+98%, P = 0.005), DPD (+42%, P = 0.013), pentosidine (+55-fold, P = 0.005), and the degree of type I collagen C-telopeptide isomerization (+4.9-fold, P = 0.005) [7].
• Mn++ complexed to DPDP (N,N'-dipyridoxylethylenediamine-N,N'-diacetate-5,5'-bis(phosphate) generic name: mangafodipir), abbreviated MnDPDP, acts as an effective contrast enhancing agent for liver MRI [8].

Analytical, diagnostic and therapeutic context of DPYD

• In the present study the molecular cloning of DPD with isolation of a cDNA coding for bovine liver DPD is reported using polymerase chain reaction (PCR) methodology [4].

References