High impact information on SERPINE2

• In contrast, fibroblast growth factor-2 inhibited tPA secretion and SERPINE2 secretion and expression [1].
• In this study, we hypothesized that PA and SERPINE2 expression/secretion by granulosa cells are regulated by FSH and growth factors [1].
• One PA inhibitor, serine protease inhibitor-E2 (SERPINE2) is expressed in granulosa but not theca cells, and expression changes with follicle development [1].
• Estradiol and SERPINE2 secretion were highly positively correlated, but estradiol did not alter SERPINE2 expression [1].
• SERPINE2 mRNA and protein levels, tPA gene expression and uPA secretion were stimulated by FSH [1].

Biological context of SERPINE2

• Immunoblotting with a specific antibody raised against a fragment of SERPINE2 (S(12)-R(196)) showed that SERPINE2 migrated at 47.5 kDa in support of glycosylation [2].
• This report demonstrates that SERPINE2 mRNA is regulated in a spatio-temporal pattern with highest levels in granulosa cells of growing dominant bovine follicles, and support the hypothesis that a high expression of SERPINE2 may contribute to follicular growth whereas a decrease following hCG injection may contribute to ovulation [2].

Associations of SERPINE2 with chemical compounds

• As estradiol is a known marker of follicle health, and SERPINE2 is an anti-apoptotic factor, we propose that SERPINE2 is involved in the regulation of atresia in bovine follicles [1].

Regulatory relationships of SERPINE2

• Epidermal growth factor inhibited SERPINE2 secretion and expression, but increased secreted tPA activity [1].

Other interactions of SERPINE2

• Detection of previously identified genes such as CX43, CYP19, INHBA, and SERPINE2 supported the validity of our experimental model and the use of SSH as the method of analysis [3].

Analytical, diagnostic and therapeutic context of SERPINE2

• Semi-quantitative real-time fluorescent RT-PCR showed a six-fold increase (P = 0.0002) in mRNA level of SERPINE2 in granulosa cells of D5 dominant follicle compared to granulosa cells collected from the <or=4 mm or preovulatory hCG-induced follicles [2].

References