High impact information on His2B:CG17949

• A 3-kilobase DNA segment characteristic of a transposable element was found within a histone H2B pseudogene in a higher eukaryote, the sea urchin Stronglyocentrotus purpuratus [1].
• This heat-shock polypeptide was identified as histone H2b by two criteria: (a) it comigrated with authentic histone H2b in Triton-urea-acetic acid acrylamide gel electrophoresis after solubilization from nuclei with acid; and (b) partial proteolysis peptide maps of the basic heat-shock protein and histone H2b were identical [2].
• The Drosophila nuclear lamina protein YA binds to DNA and histone H2B with four domains [3].
• These stress-induced changes in the methylation level of the N-terminal proline residue of histone H2B and shift in the methylation sites of histone H3 may be involved in the restructuration of chromatin accompanying the inactivation of normal genes in response to stress [4].
• Both types of stress induce a rapid increase in the methylation level of histone H2B [4].

Biological context of His2B:CG17949

• As such, Paf1 is directly involved in transcription elongation via histone H2B ubiquitination and histone H3 methylation [5].
• TAF1 activates transcription by phosphorylation of serine 33 in histone H2B [6].
• By using a DNase I footprinting assay, we have purified a factor by DNA affinity chromatography that binds to the minimal enhancer region of the Drosophila knirps gene and subsequently identified the protein as the core histone H2B [7].
• The complete amino acid sequence of histone H2B from the nematode Caenorhabditis elegans was determined [8].
• The polyadenylation of replication-dependent histone H2B, H3 and H4 mRNAs in Drosophila melanogaster was analysed [9].

Associations of His2B:CG17949 with chemical compounds

• The Km for activation by cAMP is substrate-dependent with Km values of 20 nM with histone H2B and 100 nM with the peptide, Leu-Arg-Arg-Ala-Ser-Leu-Gly [10].

Enzymatic interactions of His2B:CG17949

• Here we report that histone H2B is phosphorylated at evolutionarily conserved Ser33 (H2B-S33) by the carboxyl-terminal kinase domain (CTK) of the Drosophila TFIID subunit TAF1 [6].

Other interactions of His2B:CG17949

• YA's binding to DNA and histone H2B is mediated by four domains distributed along the length of the YA molecule [3].

Analytical, diagnostic and therapeutic context of His2B:CG17949

• Semi-quantitative RT-PCR indicated that after heat-shock treatment, the expression levels of histone H3, histone H2B and TCTP increased 4.8, 27 and 5.7-fold, respectively [11].

References