High impact information on dock

• A Drosophila homolog of human Down syndrome cell adhesion molecule (DSCAM), an immunoglobulin superfamily member, was isolated by its affinity to Dock, an SH3/SH2 adaptor protein required for axon guidance [1].
• Pak functions downstream of Dock to regulate photoreceptor axon guidance in Drosophila [2].
• We propose Dock transmits signals in the growth cone in response to guidance and targeting cues [3].
• Dock protein contains one SH2 and three SH3 domains, implicating it in tyrosine kinase signaling, and is highly related to the human proto-oncogene Nck [3].
• The signaling cascade linking the action of Dock/Nck to the reorganization of cytoskeleton is poorly understood [4].

Biological context of dock

• Detailed analyses of the mutant phenotypes in whole mutants and in small multicellular clones indicate that Dock and Pak do not control olfactory neuron (ON) differentiation, but specifically regulate multiple aspects of axon trajectories to guide them to their cognate glomeruli [5].
• Tyrosine phosphorylation of Dscam recruits the Src homology 2 domain of the adaptor protein Dock to the receptor [6].
• The Drosophila adapter, Dreadlocks (Dock), the homolog of mammalian Nck containing three N-terminal SH3 domains and a single SH2 domain, is highly specialized for growth-cone guidance [7].
• The Dmel/Nck gene maps to chromosome 3 (93A-B) using in situ hybridization to polytene chromosomes, the same position as the Na(+)-K(+)-ATPase, a related transporter [8].
• Alternative splicing for the Dmel/Nck gene is suggested in the same intracellular loop region as demonstrated for NCX1 [8].

Anatomical context of dock

• We now show that Msn likewise interacts with Dreadlocks (Dock), the Drosophila homolog of Nck. dock is required for the correct targeting of photoreceptor axons [9].
• Concomitant loss of both maternally and zygotically derived Dock dramatically enhances these central nervous system defects, but does not increase the delay in RP3 synaptogenesis [10].
• These results indicate that Dock facilitates synapse formation by the RP3 motoneuron and is also required for guidance of some interneuronal axons The involvement of Dock in the conversion of the RP3 growth cone into a presynaptic terminal may reflect a role for Dock-mediated signaling in remodeling of the growth cone's cytoskeleton [10].

Associations of dock with chemical compounds

• We report here that the SH2/SH3 adapter Dock and the serine/threonine kinase Pak are necessary for the precise guidance of olfactory axons [5].

Physical interactions of dock

• We now demonstrate that Dock interacts with the Ste20-like kinase Misshapen (Msn) in the Drosophila photoreceptor (R cell) growth cones [4].

Other interactions of dock

• Pak signaling within this pathway is specified by a required interaction with the adaptor protein Dreadlocks (Dock) [11].
• DInR functions as a guidance receptor for the adapter protein Dock/Nck [12].
• Lamellae are initiated by parallel and partially redundant signaling pathways involving Rac GTPases and the adaptor protein Nck, which stimulate SCAR, an Arp2/3 activator [13].

Analytical, diagnostic and therapeutic context of dock

• Immune precipitations of Dock also co-precipitate two tyrosine-phosphorylated proteins having molecular masses of 190 and 145 kDa [14].

References