High impact information on capu

• Premature microtubule-dependent cytoplasmic streaming in cappuccino and spire mutant oocytes [1].
• This suggests that the premature cytoplasmic streaming in capu mutant oocytes does not suffice to explain the patterning defects [2].
• In addition, capu shares both a proline-rich region and a 70-amino-acid domain with a number of other genes, two of which also function in pattern formation, the Saccharomyes cerevisiae BNI1 gene and the Aspergillus FigA gene [2].
• Germ-line mosaics indicate that cappuccino and spire are required in the oocyte-nurse cell complex [3].
• Coordination of microtubule and microfilament dynamics by Drosophila Rho1, Spire and Cappuccino [4].

Biological context of capu

• This, together with the similarity of mutant phenotypes, suggests that profilin and CAPPUCCINO may interact during development [5].
• Soma-to-germline interactions during Drosophila oogenesis are influenced by dose-sensitive interactions between cut and the genes cappuccino, ovarian tumor and agnostic [6].
• Egg chambers produced by cut, cappuccino, and chickadee mutants contained binucleate cells in which ring canal remnants stained with antibodies against Hu-li tai shao and Kelch, two proteins that are added to ring canals after cytokinesis is complete [6].

Anatomical context of capu

• We propose that Rho1, cappuccino and spire are elements of a conserved developmental cassette that is capable of directly mediating crosstalk between microtubules and microfilaments [4].
• Here, we demonstrate that Capu and Spire have microtubule and microfilament crosslinking activity [4].

Other interactions of capu

• Consistent with its roles in regulating actin cytoskeletal organization, we find that Rho1 interacts genetically and physically with the Drosophila formin homologue, cappuccino [7].
• We have investigated the role of the actin cytoskeleton during mid-oogenesis and have found that disrupting the actin cytoskeleton with cytochalasin D induces microtubule bundling and microtubule-based cytoplasmic streaming within the oocyte, similar to that which occurs prematurely in cappuccino and spire mutant oocytes [5].
• These results demonstrate that a signaling pathway regulating the morphology of germline cells is sensitive to genetic doses of cut and the genes cappuccino, ovarian tumor, and agnostic [6].

References