Disease relevance of Dh31

• The diuretic peptide leucokinin is known to increase fluid secretion in Malpighian tubules of the yellow fever mosquito Aedes aegypti by increasing a transepithelial Cl(-) conductance [1].

High impact information on Dh31

• CG17415 antibodies revealed expression within the principal cells of Malpighian tubules, further implicating DH31 as a ligand for this receptor [2].
• Using an antibody raised against D. punctata (Dippu) DH31, we demonstrate the presence of Dippu-DH31-like immunoreactivity in the CNS, salivary glands, hindgut and neurohemal sites of 5th instar Rhodnius [3].
• The identification of a Drosophila receptor for the DH neuropeptide provides a basis for genetic analysis of this critical factor's roles in maintaining physiological homeostasis [4].
• When expressed in mammalian cells, CG8422 conferred selective sensitivity to DH, as indicated by translocation of a beta-arrestin-2-GFP reporter from the cytoplasm to the cell membrane [4].
• The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP [5].

Biological context of Dh31

• The Drosophila melanogaster homologue of an insect calcitonin-like diuretic hormone was identified in a BLAST search of the Drosophila genome database [6].

Anatomical context of Dh31

• The predicted 31-residue amidated peptide (D. melanogaster DH31; Drome-DH31) was synthesised and tested for activity on fruit fly Malpighian tubules [6].

Associations of Dh31 with chemical compounds

• Taken together, the data are consistent with Drome-DH31 activating an apical membrane V-ATPase via cyclic AMP [6].
• Exogenous 8-bromo-cyclic AMP and cyclic GMP increased tubule secretion to the same extent as Drome-DH31 and, when tested together with the diuretic peptide, their activities were not additive [6].
• Stimulation with Drome-DH31 resulted in a dose-dependent increase in cyclic AMP production by tubules incubated in saline containing 0.5 mmol x l(-1) 3-isobutyl-1-methylxanthine, whereas cyclic GMP production was unchanged [6].
• The role of 5-HT as a diuretic hormone in adults has been questioned [7].

Regulatory relationships of Dh31

• The Drosophila melanogaster homologue of an insect calcitonin-like diuretic peptide stimulates V-ATPase activity in fruit fly Malpighian tubules [6].

Other interactions of Dh31

• These cells also express receptors for leucokinin, another major diuretic peptide, implying that the cells may be important in homeostatic regulation [5].

References