Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

INPP5B - inositol polyphosphate-5-phosphatase, 75kDa

Homo sapiens

Synonyms: 5PTase, 75 kDa inositol polyphosphate-5-phosphatase, OCRL2, Phosphoinositide 5-phosphatase, Type II inositol 1,4,5-trisphosphate 5-phosphatase

Jackson, S.P. et al., Speed, C.J. et al., Ono, A. et al., Jänne, P.A. et al., Varnai, P. et al., et al.

Ono, Ablan, Lockett, Nagashima, Freed,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of INPP5B

To explore the possibility that this lipid may be involved in Gag targeting and virus particle production, we overexpressed phosphoinositide 5-phosphatase IV, an enzyme that depletes cellular PI(4,5)P2, or overexpressed a constitutively active form of Arf6 (Arf6/Q67L), which induces the formation of PI(4,5)P2-enriched endosomal structures [1].

High impact information on INPP5B

Rapamycin (rapa)-induced heterodimerization of the FRB domain of the mammalian target of rapa and FKBP12 was used to translocate a phosphoinositide 5-phosphatase (5-ptase) enzyme to the plasma membrane (PM) to evoke rapid changes in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) levels [2].
One of these enzymes is the 75 kDa inositol polyphosphate 5-phosphatase (75 kDa 5-phosphatase), which has previously been demonstrated to metabolize inositol 1,4,5-trisphosphate [Ins(1,4,5)P3], inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] [3].
Phosphatidylinositol 3,4,5-trisphosphate is a substrate for the 75 kDa inositol polyphosphate 5-phosphatase and a novel 5-phosphatase which forms a complex with the p85/p110 form of phosphoinositide 3-kinase [3].
The gene product (ocrl-1) has extensive sequence homology to a 75 kDa inositol polyphosphate 5-phosphatase [4].
The 75-kDa inositol polyphosphate 5-phosphatase (5-phosphatase II) hydrolyzes various signaling molecules including the following: inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate, and phosphatidylinositol 3,4, 5-trisphosphate [5].

Biological context of INPP5B

This map position is within the conserved linkage group corresponding to the short arm of human Chromosome 1, where the human homologue, INPP5B, has been shown to map previously [6].
Mapping of the 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b) to distal mouse chromosome 4 and its exclusion as a candidate gene for dysgenetic lens [6].
The catalytic properties of the type II phosphoinositide 5-phosphatases of Lowe's oculocerebrorenal syndrome, INPP5B, Synaptojanin1, Synaptojanin2 and SKIP were analysed with respect to their substrate specificity and enzymological properties [7].
Tissue distribution and intracellular localisation of the 75-kDa inositol polyphosphate 5-phosphatase [8].
The 75-kDa inositol polyphosphate 5-phosphatase (75-kDa 5-phosphatase) hydrolyses several important mediators of intracellular calcium homeostasis, including inositol 1,4,5-trisphosphate [Ins(1,4,5)P3], inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] [8].

Associations of INPP5B with chemical compounds

We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphatase II) [9].

References

Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane. Ono, A., Ablan, S.D., Lockett, S.J., Nagashima, K., Freed, E.O. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells. Varnai, P., Thyagarajan, B., Rohacs, T., Balla, T. J. Cell Biol. (2006) [Pubmed]
Phosphatidylinositol 3,4,5-trisphosphate is a substrate for the 75 kDa inositol polyphosphate 5-phosphatase and a novel 5-phosphatase which forms a complex with the p85/p110 form of phosphoinositide 3-kinase. Jackson, S.P., Schoenwaelder, S.M., Matzaris, M., Brown, S., Mitchell, C.A. EMBO J. (1995) [Pubmed]
Lowe syndrome, a deficiency of phosphatidylinositol 4,5-bisphosphate 5-phosphatase in the Golgi apparatus. Suchy, S.F., Olivos-Glander, I.M., Nussabaum, R.L. Hum. Mol. Genet. (1995) [Pubmed]
Distinct membrane and cytosolic forms of inositol polyphosphate 5-phosphatase II. Efficient membrane localization requires two discrete domains. Matzaris, M., O'Malley, C.J., Badger, A., Speed, C.J., Bird, P.I., Mitchell, C.A. J. Biol. Chem. (1998) [Pubmed]
Mapping of the 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b) to distal mouse chromosome 4 and its exclusion as a candidate gene for dysgenetic lens. Jänne, P.A., Rochelle, J.M., Martin-DeLeon, P.A., Stambolian, D., Seldin, M.F., Nussbaum, R.L. Genomics (1995) [Pubmed]
Type II phosphoinositide 5-phosphatases have unique sensitivities towards fatty acid composition and head group phosphorylation. Schmid, A.C., Wise, H.M., Mitchell, C.A., Nussbaum, R., Woscholski, R. FEBS Lett. (2004) [Pubmed]
Tissue distribution and intracellular localisation of the 75-kDa inositol polyphosphate 5-phosphatase. Speed, C.J., Matzaris, M., Bird, P.I., Mitchell, C.A. Eur. J. Biochem. (1995) [Pubmed]
Mutation of the conserved domains of two inositol polyphosphate 5-phosphatases. Jefferson, A.B., Majerus, P.W. Biochemistry (1996) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

INPP5B	Bos taurus
ocrl-1	Caenorhabditis elegans
INPP5B	Canis lupus familiaris
inpp5b	Danio rerio
INPP5B	Gallus gallus
Inpp5b	Mus musculus
Os09g0394600	Oryza sativa Japonica Group
Os08g0425500	Oryza sativa Japonica Group
INPP5B	Pan troglodytes
Inpp5b	Rattus norvegicus
LOC100497120	Xenopus (Silurana) tropicalis

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.