Disease relevance of cid

• Further, we demonstrate that divergent cid alleles are not sufficient to cause inviability or female sterility in hybrid crosses [1].

High impact information on cid

• However, dramatic new results in mammalian and Drosophila cells show that CENP-A deposition is uncoupled from the replication of centromere DNA [2].
• Finally, a new report provides a glimpse into the potential regulation of CENP-A through specific post-translational phosphorylation, suggesting a broad level of control through histone tail modifications [2].
• We conclude that the centromere and flanking heterochromatin are physically and functionally separable protein domains that are required for different inheritance functions, and that CID is required for normal kinetochore formation and function, as well as cell-cycle progression [3].
• The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions [3].
• Injection of CID antibodies into early embryos, as well as RNA interference in tissue-culture cells, showed that CID is required for several mitotic processes [3].

Biological context of cid

• Here we show that CID, the Drosophila homologue of the CENP-A centromere-specific H3-like proteins, colocalizes with molecular-genetically defined functional centromeres in minichromosomes [3].
• We conclude that CID mislocalization promotes formation of ectopic centromeres and multicentric chromosomes, which causes chromosome missegregation, aneuploidy, and growth defects [4].
• Analysis of mitoses in living and fixed cells reveals that mitotic delays, anaphase bridges, chromosome fragmentation, and cell and organismal lethality are all direct consequences of CID mislocalization [4].
• Thus, CENP-A mislocalization is one possible mechanism for genome instability during cancer progression, as well as centromere plasticity during evolution [4].
• However, transiently expressed CENP-A incorporates throughout chromatin indicating that CENP-A nucleosomes can also be promiscuously deposited during DNA replication [5].

Other interactions of cid

• The centromere-specific histone H3 variant CENP-A plays a crucial role in kinetochore specification and assembly [6].
• Drosophila CENP-A mutations cause a BubR1-dependent early mitotic delay without normal localization of kinetochore components [7].

References