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Gene Review

repU  -  plasmid replication protein

Staphylococcus saprophyticus

 
 
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Disease relevance of repU

  • A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110 [1].
  • These results suggest that pT181, the prototype of an entire class of plasmids from Gram-positive bacteria, responds to overexpression of its replication initiator by a decrease in plasmid replication [2].
  • Growth of Staphylococcus aureus strains containing the temperature-sensitive recombinant (pGO161) at the nonpermissive temperature for plasmid replication (42 degrees C) revealed no translocation of Gmr from its plasmid location [3].
 

High impact information on repU

  • This suggests that normal plasmid replication requires primosome assembly and that the primosome can assemble not only at ssiA but also elsewhere on the plasmid [4].
  • The existence of a diffusible pT181 product required for plasmid replication has been proposed on the basis of trans-complementable thermosensitive mutants defective in plasmid maintenance (phenotype Tsr) [5].
  • The Staphylococcus aureus homologue, CBF1, which had been characterized as a double-stranded DNA binding protein involved in plasmid replication, was also shown to be an Mn(2+)-dependent exoribonuclease [6].
  • Binding of a novel host factor to the pT181 plasmid replication enhancer [7].
  • Cloning of DNA into the HindIII site of pCW8 and the HindIII and EcoRI sites of pC221 did not disrupt either plasmid replication of Cm resistance [8].
 

Biological context of repU

 

Analytical, diagnostic and therapeutic context of repU

  • Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein [12].

References

  1. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Leclère, V., Béchet, M., Adam, A., Guez, J.S., Wathelet, B., Ongena, M., Thonart, P., Gancel, F., Chollet-Imbert, M., Jacques, P. Appl. Environ. Microbiol. (2005) [Pubmed]
  2. Plasmid pT181 replication is decreased at high levels of RepC per plasmid copy. Iordanescu, S. Mol. Microbiol. (1995) [Pubmed]
  3. Mobility of gentamicin resistance genes from staphylococci isolated in the United States: identification of Tn4031, a gentamicin resistance transposon from Staphylococcus epidermidis. Thomas, W.D., Archer, G.L. Antimicrob. Agents Chemother. (1989) [Pubmed]
  4. Primosome assembly site in Bacillus subtilis. Bruand, C., Ehrlich, S.D., Jannière, L. EMBO J. (1995) [Pubmed]
  5. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication. Novick, R.P., Adler, G.K., Majumder, S., Khan, S.A., Carleton, S., Rosenblum, W.D., Iordanescu, S. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  6. Bacillus subtilis YhaM, a member of a new family of 3'-to-5' exonucleases in gram-positive bacteria. Oussenko, I.A., Sanchez, R., Bechhofer, D.H. J. Bacteriol. (2002) [Pubmed]
  7. Binding of a novel host factor to the pT181 plasmid replication enhancer. Zhang, Q., Soares de Oliveira, S., Colangeli, R., Gennaro, M.L. J. Bacteriol. (1997) [Pubmed]
  8. Characterization and construction of molecular cloning vehicles within Staphylococcus aureus. Wilson, C.R., Baldwin, N.J. J. Bacteriol. (1978) [Pubmed]
  9. Characterization of a novel type of MLSB resistance plasmid from Staphylococcus saprophyticus carrying a constitutively expressed erm(C) gene. Hauschild, T., Lüthje, P., Schwarz, S. Vet. Microbiol. (2006) [Pubmed]
  10. Specificity of the interactions between the Rep proteins and the origins of replication of Staphylococcus aureus plasmids pT181 and pC221. Iordanescu, S. Mol. Gen. Genet. (1989) [Pubmed]
  11. Plasmid replication in a temperature-sensitive chromosome replication mutant of Staphylococcus aureus. Summers, D.K., Dyke, K.G. J. Gen. Microbiol. (1983) [Pubmed]
  12. Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein. Klimenko, D.E., Convery, M.A., Rowsell, S., Thomas, C.D., Phillips, S.E. Acta Crystallogr. D Biol. Crystallogr. (1999) [Pubmed]
 
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