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Gene Review

repU  -  plasmid replication protein

Staphylococcus saprophyticus

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Disease relevance of repU

  • A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110 [1].
  • These results suggest that pT181, the prototype of an entire class of plasmids from Gram-positive bacteria, responds to overexpression of its replication initiator by a decrease in plasmid replication [2].
  • Growth of Staphylococcus aureus strains containing the temperature-sensitive recombinant (pGO161) at the nonpermissive temperature for plasmid replication (42 degrees C) revealed no translocation of Gmr from its plasmid location [3].

High impact information on repU

  • This suggests that normal plasmid replication requires primosome assembly and that the primosome can assemble not only at ssiA but also elsewhere on the plasmid [4].
  • The existence of a diffusible pT181 product required for plasmid replication has been proposed on the basis of trans-complementable thermosensitive mutants defective in plasmid maintenance (phenotype Tsr) [5].
  • The Staphylococcus aureus homologue, CBF1, which had been characterized as a double-stranded DNA binding protein involved in plasmid replication, was also shown to be an Mn(2+)-dependent exoribonuclease [6].
  • Binding of a novel host factor to the pT181 plasmid replication enhancer [7].
  • Cloning of DNA into the HindIII site of pCW8 and the HindIII and EcoRI sites of pC221 did not disrupt either plasmid replication of Cm resistance [8].

Biological context of repU


Analytical, diagnostic and therapeutic context of repU

  • Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein [12].


  1. Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Leclère, V., Béchet, M., Adam, A., Guez, J.S., Wathelet, B., Ongena, M., Thonart, P., Gancel, F., Chollet-Imbert, M., Jacques, P. Appl. Environ. Microbiol. (2005) [Pubmed]
  2. Plasmid pT181 replication is decreased at high levels of RepC per plasmid copy. Iordanescu, S. Mol. Microbiol. (1995) [Pubmed]
  3. Mobility of gentamicin resistance genes from staphylococci isolated in the United States: identification of Tn4031, a gentamicin resistance transposon from Staphylococcus epidermidis. Thomas, W.D., Archer, G.L. Antimicrob. Agents Chemother. (1989) [Pubmed]
  4. Primosome assembly site in Bacillus subtilis. Bruand, C., Ehrlich, S.D., Jannière, L. EMBO J. (1995) [Pubmed]
  5. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication. Novick, R.P., Adler, G.K., Majumder, S., Khan, S.A., Carleton, S., Rosenblum, W.D., Iordanescu, S. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  6. Bacillus subtilis YhaM, a member of a new family of 3'-to-5' exonucleases in gram-positive bacteria. Oussenko, I.A., Sanchez, R., Bechhofer, D.H. J. Bacteriol. (2002) [Pubmed]
  7. Binding of a novel host factor to the pT181 plasmid replication enhancer. Zhang, Q., Soares de Oliveira, S., Colangeli, R., Gennaro, M.L. J. Bacteriol. (1997) [Pubmed]
  8. Characterization and construction of molecular cloning vehicles within Staphylococcus aureus. Wilson, C.R., Baldwin, N.J. J. Bacteriol. (1978) [Pubmed]
  9. Characterization of a novel type of MLSB resistance plasmid from Staphylococcus saprophyticus carrying a constitutively expressed erm(C) gene. Hauschild, T., Lüthje, P., Schwarz, S. Vet. Microbiol. (2006) [Pubmed]
  10. Specificity of the interactions between the Rep proteins and the origins of replication of Staphylococcus aureus plasmids pT181 and pC221. Iordanescu, S. Mol. Gen. Genet. (1989) [Pubmed]
  11. Plasmid replication in a temperature-sensitive chromosome replication mutant of Staphylococcus aureus. Summers, D.K., Dyke, K.G. J. Gen. Microbiol. (1983) [Pubmed]
  12. Crystallization and preliminary X-ray crystallographic studies of RepDC, a hybrid rolling-circle plasmid replication initiator protein. Klimenko, D.E., Convery, M.A., Rowsell, S., Thomas, C.D., Phillips, S.E. Acta Crystallogr. D Biol. Crystallogr. (1999) [Pubmed]
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