Disease relevance of BRAF

• Complete nucleotide sequence of IC10, a retrovirus containing the Rmil oncogene transduced in chicken neuroretina cells infected with avian retrovirus RAV-1 [1].

High impact information on BRAF

• NR cell multiplication results from the frequent activation and subsequent retroviral transduction of two related serine/threonine protein kinases, the c-mil/c-raf or c-Rmil/B-raf genes [2].
• c-Rmil is the cellular allele of the v-Rmil oncogene transduced during in vitro passaging of Rous-associated virus type 1 in chicken embryonic neuroretina (NR) cells [3].
• Two c-Rmil cDNA species, resulting from an alternative splicing mechanism, were isolated from quail embryonic NR cDNA libraries [3].
• Expression of these cDNAs in COS-1 cells leads to the synthesis of two proteins with apparent molecular weights of 93.5 and 95 kDa, recognized by an Rmil-specific antiserum [3].
• We previously reported that serial passaging of Rous-associated virus type 1 in nondividing chicken embryo neuroretina cells leads to reproducible generation of acutely mitogenic retroviruses that transduced the catalytic domain of c-mil/c-raf or c-Rmil/B-raf [4].

Biological context of BRAF

• c-Rmil is the cellular allele of the v-Rmil oncogene, transduced during in vitro passaging of Rous associated virus type 1 in chicken embryonic neuroretina (NR) cells [5].
• We show that c-Rmil proteins are encoded by 19 exons lying within about 100 kbp of genomic DNA [5].
• We recently reported that the avian c-Rmil gene encodes two proteins of 94 and 95 kDa, resulting from an alternative splicing mechanism [5].
• The 3' ends of both viruses were generated by recombination between Rmil and env genes, involving partial sequence identity [6].

Associations of BRAF with chemical compounds

• Both IC11 and IC10 transduced only the serine/threonine kinase domain of c-Rmil [6].

References