Disease relevance of RCJMB04_7g5

• GroEL/GroES, the Hsp60 homolog of E. coli, increased considerably the yield of renaturation of mitochondrial aspartate aminotransferase and to a lesser extent that of its cytosolic counterpart, but not that of the E. coli enzyme [1].
• The identification of two unique SNPs closely located at positions 291 and 294 of the hsp60 gene enabled the differentiation of the three Campylobacter species [2].
• We used qPCR and the target gene chaperonin-60 (cpn60) to enumerate Clostridium perfringens genomes in DNA extracts from contents of the chicken gastrointestinal tract with the aim of optimizing this methodology to enumerate any bacterium of interest [3].
• Hsp60 specific antibodies in egg yolks from laying hens naturally infected with Salmonella enterica subspecies enterica serovar Enteritidis [4].

High impact information on RCJMB04_7g5

• Other molecular chaperones, the heat-shock proteins Hsp40, Hsp60 and Hsp90, did not seem to compensate the loss of Hsc70 [5].
• Using cells double labeled with antibodies to vimentin and the HSP 60 protein, we found that the changes in mitochondria were accompanied by perturbations of the intermediate filament network that we and others have reported previously for heat shocked cells [6].
• Anion exchange chromatography of the spiked test samples enabled accurate enumeration of C. perfringens using a standard curve comprised of a plasmid containing a fragment of C. perfringens cpn60 [3].

Anatomical context of RCJMB04_7g5

• Vital dye staining of mitochondria with rhodamine 123 and indirect immunofluorescence staining with antibodies against the mitochondrial-matrix protein, HSP 60, confirmed these results [6].

Analytical, diagnostic and therapeutic context of RCJMB04_7g5

• Eggs of laying hens from two Salmonella Enteritidis naturally infected flocks (I--acute outbreak of infection; II--occasional bacteria excretion) and one control flock (III) were tested for the presence of yolk antibodies (IgY) against Hsp60 by applying enzyme-linked immunosorbent assay (ELISA) [4].

References