High impact information on LOC443303

• Both vasoconstrictor and enzymatic activity on t-butoxycarbonyl-valine-proline-arginine-methylcoumarin amide were completely suppressed by amidinophenylmethylsulfonyl fluoride and soybean trypsin inhibitor; however, they were unaffected by hirudin, a thrombin inhibitor [1].
• The serum effects were prevented by mild heating (50 degrees C or 56 degrees C, 30 min) but not by treating serum with 10 mM EGTA and 10 mM MgCl2, or with soybean trypsin inhibitor [2].
• In addition, sMCP-1 was found to be mitogenic for bovine pulmonary artery fibroblasts, but was not mitogenic in the presence of soya-bean trypsin inhibitor [3].
• Without trypsin inhibitor, crude collagenase significantly and selectively reduced the octanoate (10 mmol.l-1)-induced amylase release [4].
• Stimulation with fatty acid as well as acetylcholine raised amylase release in a concentration-dependent manner in cells dispersed from tissue segments with crude collagenase and trypsin inhibitor [4].

Anatomical context of LOC443303

• Soyabean lectin and trypsin inhibitor appeared in the duodenum within one hour, both reached the terminal ileum and disappeared within 24 hours [5].

Associations of LOC443303 with chemical compounds

• The protease activity was inhibited by 5 X 10(-3) M p-chloromercuribenzoate and by phenylmethyl sulfonyl fluoride, TPCK, and soybean trypsin inhibitor, therefore the enzymatic activity probably depends on a serine residue and a sulfhydryl group [6].
• This reduction was abolished in mixtures in which PMSF was present together with 3 mM EGTA, 5 mM EDTA and 0.1 mg/mL trypsin inhibitor [7].

References