High impact information on sub

• In the absence of Subito, central spindle formation is defective and AurB and Incenp fail to properly localize [1].
• Subito mutants are characterized by the formation of tripolar or monopolar spindles and nondisjunction of homologous chromosomes at meiosis I [1].
• We propose that Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle [1].
• However, the role of Subito during mitotic anaphase resembles other kinesin 6 family members [2].
• Consistent with this localization pattern, subito mutants improperly assembled microtubules at metaphase, causing activation of the spindle assembly checkpoint and lagging chromosomes at anaphase [2].

Biological context of sub

• In Dub mutant females both nonexchange and exchange chromosomes undergo nondisjunction, but the effect of Dub on nonexchange chromosomes is more pronounced [3].
• When homozygous, Dub is a conditional lethal allele and exhibits phenotypes consistent with cell death [3].
• Genetic evidence suggested that the effects of subito mutants are attenuated as a result of redundant mechanisms for spindle assembly and cytokinesis [2].
• During metaphase, Subito localized to microtubules at the center of the mitotic spindle, probably interpolar microtubules that originate at the poles and overlap in antiparallel orientation [2].

Anatomical context of sub

• The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes [1].

Other interactions of sub

• subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster [4].
• Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators [2].

References