Disease relevance of Vent1

• We show that Vent DNA polymerase produces correct, blunt-ended primer extension products with substantially higher efficiency than Thermus aquaticus (Taq) DNA polymerase or modified T7 DNA polymerase (Sequenase) [1].
• The present and previous studies suggest that subarachnoid fibrosis is an important factor in the disturbance of the spatial learning ability of rats, whereas ventricular size is less important [2].

High impact information on Vent1

• Significant increases in ventricular size were documented at each stage between E10.5 and E13.5, and the ability to perform serial imaging studies over 3 days of embryonic development is described [3].
• Intracranial pressure (ICP) was approximately 5 mm Hg and ventricular size <0.3 mm(3) in control mice [4].
• Differences in left ventricular long-axis function from mice to humans follow allometric scaling to ventricular size [5].
• We hypothesized that genes underlying hydrocephalus might also modulate normal variation in ventricular size [6].
• Quantitative trait loci modulate ventricular size in the mouse brain [6].

Biological context of Vent1

• BCA, isolated from Broussonetia papyrifera Vent., inhibited iron-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC(50) of 0.63 +/- 0.03 microM [7].
• Left ventricular size and function were similar for both genotypes at baseline [8].

Anatomical context of Vent1

• Ventricular size, ultrastructural features, and sodium-potassium-adenosine triphosphatase (Na+, K(+)-ATPase) activity of the subarachnoid space were examined [2].

Associations of Vent1 with chemical compounds

• Qualitative analysis of ventricular size was performed on sections cut in the coronal plane of embryos at the 15th (E15) and 18th (E18) embryonic days, and postnatal mice aged 4 days (P4) [9].

Other interactions of Vent1

• At this time of development, aberrant behaviors that mimic certain symptoms of schizophrenia also appeared in ckr mice suggesting that structural changes in ventricular size predates the onset of psychotic-like behaviors [10].

Analytical, diagnostic and therapeutic context of Vent1

• We have developed a simplified procedure for the ligation-mediated polymerase chain reaction (LMPCR) using Thermococcus litoralis DNA polymerase (Vent DNA polymerase) [1].
• Both histological analysis and in vivo catheterization of the heart showed normal ventricular size and function [11].
• Ventricular size was not significantly different between animals given intraventricular injections of particles and albumin solution at those time points (n=4 each) [12].
• We believe this study is of importance as the first report on myocardial perfusion imaging and flow validation in in vivo mouse hearts with a left ventricular size of only 5 mm using (13)N-ammonia and PET [13].

References