High impact information on Magi2

• Thus, MAGI-2 overexpression in F9 vin(-/-) cells restored PTEN protein levels [1].
• These studies indicate that PTEN protein levels are dependent on the maintenance of beta-catenin-MAGI-2 interaction, in which vinculin plays a critical role [1].
• MAGI-2 interaction with PTEN in the cell membrane is known to prevent PTEN protein degradation [1].
• ARIP1-long had one guanylate kinase domain in the NH(2)-terminal region, followed by two WW domains and five PDZ domains (PDZ1-5) [2].
• The COOH-terminal residues of ActRIIA (ESSL) agree with a PDZ-binding consensus motif, and ARIP1 recognized the consensus sequence [2].

Biological context of Magi2

• By using yeast two-hybrid screening, we isolated a cDNA clone of ARIP1 from a mouse brain cDNA library [2].
• These findings suggest that ARIP1 has a significant role in assembling activin signaling molecules at specific subcellular sites and in regulating signal transduction in neuronal cells [2].
• We detected two forms of ARIP1, ARIP1-long and ARIP1-short, which may be produced by alternative splicing [2].

Anatomical context of Magi2

• Vinculin controls PTEN protein level by maintaining the interaction of the adherens junction protein beta-catenin with the scaffolding protein MAGI-2 [1].
• High mutant contribution chimeras expressed left-side markers such as nodal bilaterally in the lateral plate mesoderm (LPM), indicating that loss of ACVRI signaling leads to left isomerism [3].

Regulatory relationships of Magi2

• Stargazin and other transmembrane AMPA receptor regulating proteins interact with synaptic scaffolding protein MAGI-2 in brain [4].

Other interactions of Magi2

• Expression of stargazin recruited MAGI-2 to cell membranes and cell-cell contact sites in transfected HEK-293T cells dependent on the presence of the stargazin -TTPV motif [4].
• We used yeast two-hybrid screening to identify MAGI-2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) as a novel candidate interactor with the cytoplasmic C termini of the TARPs [4].

References