Disease relevance of DNAJC12

• We also assessed the effects of 17beta-estradiol (10 nM) on JDP1 mRNA expression in MCF-7 breast cancer cells [1].
• PURPOSE: To identify the genotype of 18S ribosomal RNA gene (18S rDNA, Rns) of the Acanthamoeba strains isolated from patients with keratitis in northern China. METHODS: The genus-specific primers JDP1 and JDP2 were used for the amplification of the amplimer ASA.S1 [2].

High impact information on DNAJC12

• Here we describe a novel J-domain-containing protein, RME-8, identified by such genetic analysis [3].
• The latter complex is composed of an Hsp70 localized in the intermembrane space, its interaction partner Toc12, a J-domain containing protein and the intermembrane space protein Tic22 [4].
• Here we show for the first time that JDP1 is a estrogen target gene and that its expression might be used as a marker of the ER transactivation activity and may have a predictive value for response to hormonal therapy [1].
• In this study, using quantitative real-time PCR (qPCR) we evaluated the expression pattern of the JDP1 gene in a series of 72 primary breast tumours and investigated the effects of 17beta-estradiol on the expression of the JDP1 in MCF-7 breast cancer cells [1].
• High levels of JDP1 mRNA expression were significantly associated with estrogen receptor-positive status (p=0.02) [1].

Biological context of DNAJC12

• Sequence analysis of the promoter region of the JDP1 gene revealed the presence of potential estrogen response elements (EREs), suggesting it to be under the control of estrogen action [1].
• Southern blot analysis revealed that the human JDP1 gene is present as a single copy in the human genome [5].
• Human JDP1 was mapped in silico to chromosome 10q21.1, which exhibits a conserved synteny with the central region of mouse chromosome 10 [5].
• The genotype of these strains was identified using Acanthamoeba-specific primers (JDP1 and JDP2) amplifying a part of the SSUrDNA and sequencing with an internal primer (892c) [6].

Associations of DNAJC12 with chemical compounds

• The JDP1 transcripts were found to be up-regulated in a time-dependent fashion in MCF-7 cells exposed to 17beta-estradiol treatment [1].

References