Disease relevance of Dnajc12

• NMR structure of the N-terminal J domain of murine polyomavirus T antigens. Implications for DnaJ-like domains and for mutations of T antigens [1].

High impact information on Dnajc12

• In addition to a lumenal J-domain, Mtj1p contains a single transmembrane domain and a cytosolic domain which is in close contact with translating ribosomes and appears to have the ability to modulate translation [2].
• These interactions resulted in suppression of the IKK activity in a J-domain-dependent fashion and led to the cytoplasmic retention and enhanced stability of IkappaB [3].
• This reaction requires the N-terminal binding site for pocket proteins and the J domain that binds chaperones [4].
• The N-terminal 70 residues of TAg have significant homology to the J domain of Hsp40/DnaJ and cooperate with the LXCXE motif to inactivate the pRB family [5].
• We further demonstrate that the J-domain homology region of TAg confers a growth advantage to wild-type mouse embryo fibroblasts (MEFs) but is dispensable in the case of MEFs lacking both p130 and p107 [6].

Biological context of Dnajc12

• During clathrin-mediated endocytosis Hsc70, supported by the J-domain protein auxilin, uncoats clathrin-coated vesicles [7].
• Cyclin A promoter assays showed that J domain function also contributes to large T transactivation that is independent of Rb [8].
• In contrast, an intact J domain and an intact zinc finger are required at both sites [9].
• To rigorously examine J domain phylogeny, we combined the nonparametric bootstrap with Monte Carlo simulation [10].
• Here, we report detailed mutagenesis of the polyomavirus J domain in both large T (63 mutants) and middle T (51 mutants) backgrounds [8].

Associations of Dnajc12 with chemical compounds

• It contained J domain in the NH(2) terminal region and Gly/Phe-rich domain in the middle of protein, which are typical structural domains of the DnaJ protein family [11].
• The predicted protein, named MSJ-1, is 242 amino acid residues-long, containing the fingerprinting J domain in the NH2 terminus [12].

Physical interactions of Dnajc12

• Hsc70's expected binding site on helix II of the J domain of T antigens appears to be blocked in its structure bound to tumor suppressor pRb [13].

Other interactions of Dnajc12

• This suggests that binding of Rb family members by large T is not sufficient for their inactivation and that a functional J domain is required as well [14].
• Here we report the cloning and characterization of a new J-domain protein named MmDjC7 [15].
• Interestingly, the J domain in st, which lacks its own nuclear localization signal (NLS), required nuclear localization to activate apoptin [16].
• Residues Q32, A33, Y34, H49, M52, and N56 within helix 2 and helix 3 of the large T J domain were also found to be required for Rb-dependent transactivation [8].

References