High impact information on RAB5C

• Labeling with probes to telomeres and centromeres confirmed a striking Rabl configuration in all cells, with a clear clustering of the centromeres, and cell files often maintained a common polarity through several division cycles [1].
• After germinating seeds either in the presence of 5-Azacytidine (5-AC), which leads to DNA hypomethylation, or trichostatin A (TSA), which results in histone hyperacetylation, the architecture of the interphase chromosome arms changes significantly even though the overall Rabl configuration is maintained [2].
• Simultaneous FISH with pSc200 subtelomeric tandem repeat and CCS1 centromeric sequence revealed that at pre-meiotic interphase the two domains are in a bipolar Rabl orientation in both the PMCs and tapetal cells [3].
• The polar arrangement of telomeres in interphase and meiosis. Rabl organization and the bouquet [4].
• This association pattern, also exhibited by two similar MCs in human beings, the minute Y of a marsupial and certain B chromosomes in plants, probably reflects the Rabl orientation of chromosomes in interphase [5].

Biological context of RAB5C

• RABL encodes 216 amino acids that are 86% identical to members of the RAB5 subfamily and it shows 94% homology in nucleotide sequence with RAB5C of dog [6].
• By fluorescence in situ hybridization we mapped RABL to chromosome band 17q21.2 [6].
• Here we report the isolation and analysis of a cDNA encoding a putative small GTP-binding protein, designated RABL, from a human fetal lung cDNA library [6].
• Our study confirmed that bouquet arrangement of telomeres follows the Rabl configuration [7].
• Interphase chromosomes and the Rabl configuration: does genome size matter [8]?

Anatomical context of RAB5C

• Both Rabl and non-Rabl distribution patterns of centromeres and telomeres were consistent in interphase nuclei derived from meristematic root tip cells, microspore mother cells and differentiated leaf cells [9].

Analytical, diagnostic and therapeutic context of RAB5C

• The mitotic chromosomes showed neither primary constriction nor Rabl orientation whereas the meiotic ones exhibited the typical quadripartite structure characteristic of holocentrics, supporting the assumption of holocentric chromosomes as a synapomorphy of Cuscuta subg [10].

References