High impact information on Kncn

• The N-terminal amino acid sequence of this 34,000-molecular-weight protein demonstrated that the first 40 amino acids were identical to the ribosomal L5 protein, found in the large rRNA subunit and bound to 5S RNA [1].
• Region II contains most of a central acidic region required for interaction with the L5 ribosomal protein and a putative C4 zinc finger [2].
• Based on the structure of the acetylcholine binding protein, we speculate that in AChRs L5 projects from the transmitter binding site toward the membrane along a subunit interface [3].
• We used single-channel kinetics to quantify the effects of mutations to alphaD97 and other L5 residues with respect to agonist binding (to both open and closed AChRs), channel gating (for both unliganded and fully-liganded AChRs), and desensitization [3].
• Each mouse underwent a peripheral nerve injury in which the lumbar 5 spinal segment (L5) nerve was transected [4].

Anatomical context of Kncn

• Initial characterization of kinocilin, a protein of the hair cell kinocilium [5].
• In the mature vestibular hair cells, kinocilin is still present in the kinocilium [5].
• In addition, as the kinocilium regresses from developing auditory hair cells, kinocilin begins to be expressed by the pillar cells and Deiters cells, that both contain prominent transcellular and apical bundles of microtubules [5].
• Mice were killed on day 21 and the harvested L5 spinal cord was analyzed for chemokine expression using RNAse protection assay [4].
• The lumbar 5 (L5) dorsal root ganglia (DRGs) were studied in neuropeptide tyrosine (NPY)-deficient (-/-) and wild type (+/+) mice after unilateral sciatic nerve transection using in situ hybridization and immunohistochemistry [6].

Analytical, diagnostic and therapeutic context of Kncn

• NPY was not found in L5 DRGs in -/- mice, not even after axotomy [6].

References