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Gene Review:

TPM4 - tropomyosin 4

Homo sapiens

Synonyms: TM30p1, Tropomyosin alpha-4 chain, Tropomyosin-4

Li, D.Q. et al., Wilton, S.D. et al., MacLeod, A.R. et al., Bradac, J.A. et al., Meech, S.J. et al., et al.

Kim, Eo, Joh,

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Disease relevance of TPM4

Immunohistochemical analysis in 80 breast cancer donors demonstrated a significant association of TPM4 (p = 0.002), HSP60 (p = 0.001), PDX6 (p = 0.002) but not CRAB (p = 0.113) staining with the presence of lymph node metastasis [1].
Reverse transcriptase-polymerase chain reaction and nucleotide sequence analysis revealed fusion of ALK to tropomyosin 4, an ALK fusion partner not described previously in hematologic malignancies [2].

High impact information on TPM4

The derived amino acid sequence from this clone exhibits only 82% homology with rat fibroblast tropomyosin 4 and 80% homology with human fibroblast tropomyosin TM30nm, indicating that this clone encodes a third 248-amino-acid tropomyosin isoform class [3].
TPM3-ALK and TPM4-ALK oncogenes in inflammatory myofibroblastic tumors [4].
In vitro translation of an SP6 transcript of this cDNA reveals that the protein product of the 3.0 X 10(3) base mRNA is TM30p1, one of the five proteins in human fibroblasts that have been shown to possess the physical and chemical characteristics of tropomyosin [5].
Tropomyosin (TM), a component of the thin filament of the sarcomere, is encoded by a four-member multigene family: alpha-TM, beta-TM, TPM 3, and TPM 4 [6].
We identified that the cDNAs had significant similarities to the L1M4 repeat sequence of clone RP11-57L9, clone RP11-299H13, and Alu repetitive sequence of human tropomyosin 4 mRNA [7].

Biological context of TPM4

One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19 [8].
The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads [8].
Sequence-tagged sites (STSs) were developed for the human alpha-tropomyosin gene TPM4 [8].

Analytical, diagnostic and therapeutic context of TPM4

Differential expression was confirmed for four proteins including PDX6, CRAB, TPM4, and HSP60 by real-time quantitative PCR and Western blotting analysis in our model [1].

References

Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry. Li, D.Q., Wang, L., Fei, F., Hou, Y.F., Luo, J.M., Zeng, R., Wu, J., Lu, J.S., Di, G.H., Ou, Z.L., Xia, Q.C., Shen, Z.Z., Shao, Z.M. Proteomics (2006) [Pubmed]
Unusual childhood extramedullary hematologic malignancy with natural killer cell properties that contains tropomyosin 4--anaplastic lymphoma kinase gene fusion. Meech, S.J., McGavran, L., Odom, L.F., Liang, X., Meltesen, L., Gump, J., Wei, Q., Carlsen, S., Hunger, S.P. Blood (2001) [Pubmed]
Isolation and characterization of related cDNA clones encoding skeletal muscle beta-tropomyosin and a low-molecular-weight nonmuscle tropomyosin isoform. Bradac, J.A., Gruber, C.E., Forry-Schaudies, S., Hughes, S.H. Mol. Cell. Biol. (1989) [Pubmed]
TPM3-ALK and TPM4-ALK oncogenes in inflammatory myofibroblastic tumors. Lawrence, B., Perez-Atayde, A., Hibbard, M.K., Rubin, B.P., Dal Cin, P., Pinkus, J.L., Pinkus, G.S., Xiao, S., Yi, E.S., Fletcher, C.D., Fletcher, J.A. Am. J. Pathol. (2000) [Pubmed]
Characterization of a cDNA defining a gene family encoding TM30p1, a human fibroblast tropomyosin. MacLeod, A.R., Talbot, K., Smillie, L.B., Houlker, C. J. Mol. Biol. (1987) [Pubmed]
Tropomyosin 3 increases striated muscle isoform diversity. Pieples, K., Wieczorek, D.F. Biochemistry (2000) [Pubmed]
Identification of differentially expressed genes in primary varicose veins. Kim, D.I., Eo, H.S., Joh, J.H. J. Surg. Res. (2005) [Pubmed]
Assignment of the human alpha-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization. Wilton, S.D., Lim, L., Dorosz, S.D., Gunn, H.C., Eyre, H.J., Callen, D.F., Laing, N.G. Cytogenet. Cell Genet. (1996) [Pubmed]

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TPM4	Bos taurus
TPM4	Canis lupus familiaris
tpm4a	Danio rerio

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