Disease relevance of CA7

• METHODS: Forty-eight eyes of 48 patients aged 27-82 years with retinal re-detachment due to proliferative vitreoretinopathy (PVR) grades CP1 to CA7 were included [1].

High impact information on CA7

• The flanking markers CA7 and D2S378 defined a region of approximately 6.4 cM that is likely to contain the gene(s) associated with Carney complex [2].
• PCR analysis of DNAs from human-rodent somatic cell hybrids localized the gene for CA V to human chromosome 16, the same chromosome to which CA VII has previously been mapped [3].
• In addition, factors influencing the trafficking and kinetic modulation of KCC2 as well as activation/deactivation of CAVII are obvious candidates in the ionic modulation of GABAergic responses [4].
• Like the CA VII gene, CA5 does not contain typical TATA and CAAT promoter elements in the 5' flanking region but does contain a TTTAA sequence 147 nucleotides upstream of the initiation codon [5].
• The exon-intron boundaries are found in positions identical to those determined for the previously described CA II, CA III, and CA VII genes [5].

Biological context of CA7

• Characterization of the human gene for a newly discovered carbonic anhydrase, CA VII, and its localization to chromosome 16 [6].
• The 5' flanking regions of the CA VII gene do not contain the TATA and CAAT promoter elements usually found within 100 bp upstream of transcription initiation, but do contain a TTTAA sequence 102 nucleotides upstream of the initiation codon [6].
• We have isolated an additional CA gene from a human genomic library and designated its putative product carbonic anhydrase VII (CA VII) [6].
• When the available molecular sequence information is examined, it is evident that the erythrocyte CAs of early vertebrates have active sites that are more similar to those of mammalian CA VII and II, rather than CA I [7].

Anatomical context of CA7

• Based on analysis of interspecific somatic cell hybrids, the human CA VII gene, CA7, was assigned to chromosome 16, with localization to the long arm at the q21-23 region by in situ hybridization [6].

Associations of CA7 with chemical compounds

• Topiramate, ethoxzolamide and benzolamide showed subnanomolar hCA VII inhibitory activity, whereas acetazolamide, methazolamide, dorzolamide and brinzolamide showed K(I)-s in the range of 2.1-3.5 nM [8].
• Cyanate, cyanide, and hydrogensulfite were weak hCA VII inhibitors (K(I)s in the range of 7.3-15.2mM) [9].
• Cl(-) and HCO3- showed good inhibitory activity against hCA VII (K(I)s of 0.16-1.84mM), suggesting that this enzyme is not involved in metabolons with anion exchangers or sodium bicarbonate cotransporters [9].

Other interactions of CA7

• The potential for the identification and quantification of individual branched carboxyalkyl isomeric mixtures of CA5-, CA6-, CA7-, and CA8P1EC metabolites based on corresponding dm-CA5-8P1ECs revealed the advantage of the GC-CI-MS although the detection limits in CI were clearly higher than those in EI [10].

References