High impact information on Arfrp1

• Cultured homozygous Arfrp1 null-mutant blastocysts were indistinguishable from wild-type blastocysts [1].
• In order to further analyze the function of ARFRP1, we cloned the mouse Arfrp1 gene and generated Arfrp1 null-mutant mice by gene targeting in embryonic stem cells [1].
• Embryonic lethality caused by apoptosis during gastrulation in mice lacking the gene of the ADP-ribosylation factor-related protein 1 [1].
• These data suggest that lethality of Arfrp1 knockout embryos is due to a specific disruption of protein targeting, e.g., of ARL1 and Golgin-245, to the Golgi [2].
• Knockout of Arfrp1 leads to disruption of ARF-like1 (ARL1) targeting to the trans-Golgi in mouse embryos and HeLa cells [2].

Biological context of Arfrp1

• Here we describe the organization of the Arfrp1 gene and of its promoter region [3].
• The Arfrp1 gene spans approximately 7 kb and contains 8 exons [3].

Anatomical context of Arfrp1

• In contrast, the guanine nucleotide exchange defective ARFRP1 mutant (ARFRP1-T31N) clusters within the cytosol [2].
• GTP-bound ARFRP1 (ARFRP1-Q79L mutant) is associated with Golgi membranes and co-localized with the GTPase ARL1 [2].

Analytical, diagnostic and therapeutic context of Arfrp1

• Mutational analysis and electrophoretic mobility shift assays indicated that two short cRel- and cEts1-like elements in the 5'-flanking region of Arfrp1 (-76 to -53 and -45 to -23) are critical for the regulation of Arfrp1 expression [3].

References